Apaf-1 Antibody [2E10] Cat. No.: PM-2015

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psi-iconSpecifications
HOST SPECIES:Mouse
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: A peptide corresponding to amino acids near the carboxy terminus of human Apaf1.
TESTED APPLICATIONS: ELISA, IHC-P, WB
APPLICATIONS: Apaf1 antibody can be used for detection of Apaf1 by Western blot at 0.25 - 0.5 mg/mL. A 130 kDa band should be detected. Antibody can also be used for immunohistochemistry starting at 0.5 μg/mL.

Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
POSITIVE CONTROL:1) Cat. No. 1204 - K562 Cell Lysate
 2) Cat. No. 17-004 - K-562 Cell Slide
PREDICTED MOLECULAR WEIGHT: 130 kDa

psi-iconProperties
PURIFICATION:Apaf-1 Monoclonal Antibody is immunoaffinity chromotography purified IgG.
CLONALITY:Monoclonal
ISOTYPE:IgG1
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:Apaf-1 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:Apaf-1 monoclonal antibody can be stored at -20˚C, stable for one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:APAF1
ALTERNATE NAMES:Apaf-1 Antibody [2E10] : CED4, APAF-1
ACCESSION NO.:AAC51678
PROTEIN GI NO.:2330015
GENE ID:317
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:Apaf-1 Monoclonal Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologous of the key cell death gene CED-4 in C. elegans was identified recently from human and mouse and designated Apaf1 for apoptosis protease-activating factor 1. Apaf1 binds to cytochrome c (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Apaf1 can also associate with caspase-4 and caspase-8. Apaf1 transcript is ubiquitously expressed in human tissues.
REFERENCES:1) Zou H, Henzel WJ, Liu X, et al. Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3. Cell 1997; 90:405-13.
2) Cecconi F, Alvarez-Bolado G, Meyer BI, et al. Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development. Cell 1998; 94:727-37.
3) Li P, Nijhawan D, Budihardjo I, et al. Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade. Cell 1997; 91:479-89.
4) Hu Y, Benedict MA, Wu D, et al. Bcl-XL interacts with Apaf-1 and inhibits Apaf-1-dependent caspase-9 activation. Proc. Natl. Acad. Sci. USA 1998; 95:4386-91.

ANTIBODIES FOR RESEARCH USE ONLY.

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