Apaf-1 Antibody [13F11] Cat. No.: 36-176

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psi-iconSpecifications
HOST SPECIES:Rat
SPECIES REACTIVITY: Mouse, Rat
IMMUNOGEN: Recombinant mouse Apaf-1 (aa 1-97) containing the N-terminal CARD domain.
TESTED APPLICATIONS: ELISA, ICC, IP, WB
APPLICATIONS: Immunoprecipitation: (1:1000-1:2000) . Western Blot: (1:1000-1:2000) . Optimal conditions must be determined individually for each application.
SPECIFICITY: Recognizes the CARD domain of mouse and rat Apaf-1. Does not cross-react with human Apaf-1.

psi-iconProperties
PURIFICATION:>95% (SDS-PAGE)
CLONALITY:Monoclonal
ISOTYPE:IgG2a, kappa
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:Liquid. In PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/ml
STORAGE CONDITIONS:Stable for at least 1 year after receipt when stored at -20˚C.

psi-iconAdditional Info
OFFICIAL SYMBOL:Apaf1
ALTERNATE NAMES:Apoptosis Protease-activating Factor 1
ACCESSION NO.:Q9EPV5
PROTEIN GI NO.:20137192
GENE ID:78963
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:Apaf-1 is a cytoplasmic protein that forms one of the central hubs in the apoptosis regulatory network. It contains (from the N terminal) a caspase recruitment domain (CARD), an ATPase domain (NB-ARC), few short helical domains and then several copies of the WD40 repeat domain. Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome, mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis.

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