Specifications
| HOST SPECIES: | Rat |
| SPECIES REACTIVITY: |
Mouse, Rat |
| IMMUNOGEN: |
Recombinant mouse Apaf-1 (aa 1-97) containing the N-terminal CARD domain. |
| TESTED APPLICATIONS: |
ELISA, ICC, IP, WB |
| APPLICATIONS: |
Immunoprecipitation: (1:1000-1:2000) . Western Blot: (1:1000-1:2000) . Optimal conditions must be determined individually for each application. |
| SPECIFICITY: |
Recognizes the CARD domain of mouse and rat Apaf-1. Does not cross-react with human Apaf-1. |
Properties
| PURIFICATION: | >95% (SDS-PAGE) |
| CLONALITY: | Monoclonal |
| ISOTYPE: | IgG2a, kappa |
| CONJUGATE: | Unconjugated |
| PHYSICAL STATE: | Liquid |
| BUFFER: | Liquid. In PBS containing 0.02% sodium azide. |
| CONCENTRATION: | 1 mg/ml |
| STORAGE CONDITIONS: | Stable for at least 1 year after receipt when stored at -20˚C. |
Additional Info
| OFFICIAL SYMBOL: | Apaf1 |
| ALTERNATE NAMES: | Apoptosis Protease-activating Factor 1 |
| ACCESSION NO.: | Q9EPV5 |
| PROTEIN GI NO.: | 20137192 |
| GENE ID: | 78963 |
| USER NOTE: | Optimal dilutions for each application to be determined by the researcher. |
Background and References
| BACKGROUND: | Apaf-1 is a cytoplasmic protein that forms one of the central hubs in the apoptosis regulatory network. It contains (from the N terminal) a caspase recruitment domain (CARD), an ATPase domain (NB-ARC), few short helical domains and then several copies of the WD40 repeat domain. Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome, mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. |
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