AP3M1 Antibody Cat. No.: 6393

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Chicken: (100%), Bovine: (100%)
IMMUNOGEN: AP3M1 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human AP3M1.

The immunogen is located within amino acids 340 - 390 of AP3M1.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: AP3M1 antibody can be used for detection of AP3M1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
SPECIFICITY: AP3M1 antibody may cross-react with AP3M2.
POSITIVE CONTROL:1) Cat. No. 1303 - Human Brain Tissue Lysate

psi-iconProperties
PURIFICATION:AP3M1 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:AP3M1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:AP3M1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:AP3M1
ALTERNATE NAMES:AP3M1 Antibody: AP-3 complex subunit mu-1, AP-3 adapter complex mu3A subunit
ACCESSION NO.:NP_036227
PROTEIN GI NO.:46370095
GENE ID:26985
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:AP3M1 Antibody: The AP3 complex is a heterotetramer composed of two large adaptins (AP3D1, AP3B1 or AP3B2), a medium adaptin (AP3M1 or AP3M2) and a small adaptin (APS1 or AP3S2). The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes. AP3M1 is highly homologous to AP3M2 but is the preferred binding partner to the YQRL motif from the cytosolic domain of TGN38. AP3M1 has also been shown to interact with the HIV-1 virulence protein Nef.
REFERENCES:1) Dell'Angelica EC, Ohno H, Ooi CE, et al. AP-3: an adaptor-like protein complex with ubiquitous expression. EMBO J. 1997; 16:917-28
2) Stephens DJ and Banting G. Specificity of interaction between adaptor-complex medium chains and the tyrosine-based sorting motifs of TGN38 and lgp120. Biochem. J. 1998; 335:567-72
3) Coleman SH, Van Damme N, Day JR, et al. Leucine-specific, functional interactions between human immunodeficiency virus type 1 Nef and adaptor protein complexes. J. Virol. 2005; 79:2066-78.

ANTIBODIES FOR RESEARCH USE ONLY.

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