AP2M1 Antibody Cat. No.: 5161

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psi-iconSpecifications
HOST SPECIES:Chicken
SPECIES REACTIVITY: Human, Mouse, Rat
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Bovine: (100%), Chicken: (100%)
IMMUNOGEN: AP2M1 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human AP2M1.

The immunogen is located within amino acids 360 - 410 of AP2M1.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: AP2M1 antibody can be used for detection of AP2M1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
POSITIVE CONTROL:1) Cat. No. 1305 - Human Kidney Tissue Lysate

psi-iconProperties
PURIFICATION:AP2M1 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgY
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:AP2M1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:AP2M1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:AP2M1
ALTERNATE NAMES:AP2M1 Antibody: mu2, AP50, CLAPM1, KIAA0109, AP-2 complex subunit mu, AP-2 mu chain
ACCESSION NO.:NP_004059
PROTEIN GI NO.:14917109
GENE ID:1173
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:AP2M1 Antibody: The heterotetrameric coat assembly protein complex, also known as the adaptor-related protein complex 2 (AP-2), belongs to the adaptor complexes medium subunits family. The mu 1 subunit of the AP-2 complex (AP2M1) is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. AP2M1 has also been shown to associate with the HIV-1 protein Nef, suggesting that Nef may use AP-2 complex to enhance the rate of endocytosis of both CD4 and class I MHC. AP2M1 may also play an important role in regulating the intracellular trafficking and function of cytotoxic T-lymphocyte associated (CTLA)-4 protein. At least two isoforms of AP2M1 are known to exist.
REFERENCES:1) Robinson MS. Adaptable adaptors for coated vesicles. Trends Cell Biol.2004; 14:167-74.
2) Aguilar RC, Ohno H, Roche KW, et al. Functional domain mapping of the clathrin-associated adaptor medium chains mu1 and mu J. Biol. Chem.1997; 272:27160-6.
3) Liu Q, Feng Y, and Forgac M. Activity and in vitro reassembly of the coated vesicle (H+)-ATPase requires the 50-kDa subunit of the clathrin assembly complex AP-2. J. Biol. Chem.1994; 269:31592-7.
4) Craig HM, Reddy TR, Riggs NL, et al. Interactions of HIV-1 Nef with the m subunits of adaptor protein complexes 1, 2, and 3: role of the dileucine-based sorting motif. Virology271:9-17.

ANTIBODIES FOR RESEARCH USE ONLY.

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