Anthrax Protein Detection Set Cat. No.: PSI-1811

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psi-iconSpecifications
SPECIES REACTIVITY:Human
TESTED APPLICATIONS:IF, IHC, WB
APPLICATIONS:These polyclonal antibodies can be used for detection of Anthrax PA, LF or EF proteins in bodily fluid or tissue by ELISA. Immunogenic peptides are provided as positive controls and to determine protein concentration. Each antibody will detect 10 ng of its corresponding peptide. ATR antibody can be used for detection of ATR in immunoblot, immunohistochemistry, immunoflourescence, and Immunoflourescence. applications.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
SPECIFICITY:ATR antibody will recognize all three isoforms.
IMMUNOGEN:Rabbit polyclonal antibodies were raised against peptides corresponding to amino acid sequences from each of the corresponding proteins.
psi-iconSet Contents
Catalog Number 3117Catalog number 3117 — ATR Antibody
Catalog Number 3411Catalog number 3411 — Anthrax PA Antibody
Catalog Number 3417Catalog number 3417 — Anthrax Lethal Factor Antibody
Catalog Number 3419Catalog number 3419 — Anthrax Edema Factor Antibody
Catalog Number 3411PCatalog number 3411P — Anthrax PA Peptide
Catalog Number 3417PCatalog number 3417P — Anthrax Lethal Factor Peptide
Catalog Number 3419PCatalog number 3419P — Anthrax Edema Factor Peptide
psi-iconProperties
PHYSICAL STATE:Liquid
BUFFER:PBS containing 0.02% sodium azide.
CONCENTRATION:Antibody 1 mg/mL

Peptide 200 μg/mL
STORAGE CONDITIONS:Stable at 4˚C for three months, store at -20˚C for up to one year.
psi-iconAdditional Info
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground
BACKGROUND:Anthrax infection is initiated by the inhalation, ingestion, or cutaneous contact with Bacillus anthracis endospores. B. anthracis produces three polypeptides that comprise the anthrax toxin: protective antigen (PA), lethal factor (LF), and edema factor (EF). PA binds to two related proteins on the cell surface; these are termed tumor epithelial marker 8 (TEM8)/anthrax toxin receptor (ATR) and capillary morphogenesis protein 2 (CMG2). PA is cleaved into two fragments by a furin-like protease after receptor binding. The bound fragment binds both LF and EF; the resulting complex is then endocytosed into the cell which allows the release of LF and EF into the cytoplasm. These toxins are usually sufficient to cause rapid cell death, and often the death of the infected organism. LF is the primary toxin of anthrax and functions as a highly specific protease that cleaves members of the mitogen-activated protein kinase kinase (MAPKK) family near their amino terminus, interfering with MAPK signaling and inducing apoptosis . EF is a calmodulin and Ca++-dependent adenylate cyclase responsible for the edema seen in the disease. It is thought to benefit the B. anthracis bacteria by inhibiting cells of the host immune system. The Anthrax toxin receptor (ATR) was initially discovered as the tumor endothelial marker 8 (TEM8). This protein, which exists in three isoforms (36, 40, and 60 kDa), is highly expressed in tumor vessels as well as in the vasculature of developing embryos, suggesting that it may normally play a role in angiogenesis in addition to its role as the anthrax toxin receptor.

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