| HOST SPECIES: | Chicken |
| SPECIES REACTIVITY: | Human, Mouse, Rat |
| IMMUNOGEN: | ANKLE2 antibody was raised against a 19 amino acid synthetic peptide near the center of human ANKLE2. The immunogen is located within amino acids 280 - 330 of ANKLE2. |
| TESTED APPLICATIONS: | ELISA, WB |
| APPLICATIONS: | ANKLE2 antibody can be used for detection of ANKLE2 by Western blot at 1 - 2 μg/mL. Antibody validated: Western Blot in human samples. All other applications and species not yet tested. |
| SPECIFICITY: | Three isoforms of ANKLE2 are known to exist; this antibody will detect all three. ANKLE2 antibody is predicted to not cross-react with ANKLE1. |
| POSITIVE CONTROL: | 1) Cat. No. 1313 - Human Testis Tissue Lysate |
| PREDICTED MOLECULAR WEIGHT: | Predicted: 121, 139, 145 kDa Observed: 121 kDa |
Specifications| PURIFICATION: | ANKLE2 Antibody is affinity chromatography purified via peptide column. |
| CLONALITY: | Polyclonal |
| ISOTYPE: | IgY |
| CONJUGATE: | Unconjugated |
| PHYSICAL STATE: | Liquid |
| BUFFER: | ANKLE2 Antibody is supplied in PBS containing 0.02% sodium azide. |
| CONCENTRATION: | 1 mg/mL |
| STORAGE CONDITIONS: | ANKLE2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| OFFICIAL SYMBOL: | ANKLE2 |
| ALTERNATE NAMES: | ANKLE2 Antibody: Lem4, LEMD7, KIAA0692, LEM4, Ankyrin repeat and LEM domain-containing protein 2, LEM domain-containing protein 4 |
| ACCESSION NO.: | AAH43157 |
| PROTEIN GI NO.: | 148664230 |
| GENE ID: | 23141 |
| USER NOTE: | Optimal dilutions for each application to be determined by the researcher. |
| BACKGROUND: | ANKLE2 Antibody: Ankyrins are membrane adaptor molecules that play important roles in coupling integral membrane proteins to the spectrin-based cytoskeleton network. ANKLE2 (Ankyrin repeat and LEM domain containing 2) is a member of ankyrin repeat and LEM domain containing family. It is a potential single-pass membrane protein that contains three ankyrin repeats and a Lem domain. Ankyrin repeats mediate protein-protein interactions and are among the most common structural motifs in known proteins. The function of this protein is still under investigation. |
| REFERENCES: | 1) Lee KK and Wilson KL. All in the family: evidence for four new LEM-domain proteins Lem2 (NET-25), Lem3, Lem4 and Lem5 in the human genome. Symp. Soc. Exp. Biol. 2004; 56:329-39 |
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