ANG-2 Recombinant Protein Cat. No.: 40-540

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psi-iconSpecifications
SPECIES:Human
SOURCE SPECIES:CHO cells
SEQUENCE:DAPLEYDDSV QRLQVLENIM ENNTQWLMKL ENYIQDNMKK EMVEIQQNAV QNQTAVMIEI GTNLLNQTAE QTRKLTDVEA QVLNQTTRLE LQLLEHSLST NKLEKQILDQ TSEINKLQDK NSFLEKKVLA MEDKHIIQLQ SIKEEKDQLQ VLVSKQNSII EELEKKIVTA TVNNSVLQKQ QHDLMETVNN LLTMMSTSNS AKDPTVAKEE QISFRDCAEV FKSGHTTNGI YTLTFPNSTE EIKAYCDMEA GGGGWTIIQR REDGSVDFQR TWKEYKVGFG NPSGEYWLGN EFVSQLTNQQ RYVLKIHLKD WEGNEAYSLY EHFYLSSEEL NYRIHLKGLT GTAGKISSIS QPGNDFSTKD GDNDKCICKC SQMLTGGWWF DACGPSNLNG MYYPQRQNTN KFNGIKWYYW KGSGYSLKAT TMMIRPADFH HHHHH
TESTED APPLICATIONS:
BIOLOGICAL ACTIVITY: Biological activity was determined by its ability to stimulate tubulogenesis in HUVEC cells using a concentration of 0.2 μg/mL. Endotoxin level is less than 0.1 ng per μg (1EU/μg).
psi-iconProperties
PURITY:Greater than 95% by SDS-PAGE gel and HPLC analyses.
PHYSICAL STATE:Lyophilized
STORAGE CONDITIONS:The lyophilized ANG-2 recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted ANG-2 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing Yeast ANG-2 recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
psi-iconAdditional Info
ALTERNATE NAMES:ANG2, AGPT2, Angiopoietin-2, ANG-2
ACCESSION NO.:NP_001112359.1
PROTEIN GI NO.:169646749
OFFICIAL SYMBOL:ANGPT2
GENE ID:285
psi-iconBackground and References
BACKGROUND:ANG-2 binds to the endothelial cell specific receptor Tie2, but, in contrast to ANG-1 does not induce tyrosine phosphorylation. Consequently, ANG-2 modulates ANG-1 activation of Tie2 and, depending on the physiological and biochemical environment, can act either as a n agonist or antagonist of Tie2 induced angiogenesis. The signaling interactions of ANG-1, ANG-2 and Tie2, along with less characterized ANG-3 and ANG-4, are required for embryonic and adult angiogenesis. Physiologically, ANG-1 and ANG-2 are associated with sprouting, tube formation, and structural integrity of newly formed blood vessels. Mature human ANG-2 is a secreted protein containing 480 amino acid residues. ANG-2 is composed of an alpha helix rich "coiled coil” N-terminal domain and fibrinogen like C-terminal domain. ANG-2 exists predominantly in the form of a disulfide-linked dimer. Recombinant human ANG-2 is a C-terminal histidine tagged glycoprotein which migrates with an apparent molecular mass of 60.0 - 70.0 kDa by SDS-PAGE under reducing conditions. Sequencing analysis shows an N-terminal sequence starting with residue 68 (D) of the ANG-2 precursor protein.

FOR RESEARCH USE ONLY.

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