Androgen Receptor Antibody Cat. No.: 45-010

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psi-iconSpecifications
HOST SPECIES:Goat
SPECIES REACTIVITY: Human
HOMOLOGY: Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
IMMUNOGEN: The immunogen for this antibody is: EVQLGLGRVYPRPPSC
TESTED APPLICATIONS: ChIP, ELISA, IF, IHC, WB
APPLICATIONS: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 100kDa band observed in Human Cerebellum lysates (calculated MW of 99.2kDa according to NP_000035.2). Recommended concentration:0.1-0.5ug/ml. Primary incubation 1 hour at room temperature. Negative Control: Human Pancreas lysate. Preliminary testing was unsuccessful on Mouse and Rat for this particular batch.Immunohistochemistry:In paraffin embedded Human Prostate shows nuclear staining in the secretory cells of the gland. Recommended concentration: 2-3ug/ml.Immunofluorescence: Strong expression of the protein seen in the mitochondria/cytoplasm of MCF7 and U2OS cells. Recommended concentration: 10ug/ml.

Chromatin Immunoprecipitation: A six-fold increased presence of the FKBP5 locus observed when comparing the precipitations using EB06441 with non-specific goat IgG to bring down Androgen Receptor in a chromatin lysate from DHT-stimulated HEC50 cells.

SPECIFICITY: This antibody is expected to recognize isoform 1 (NP_000035.2) only.
POSITIVE CONTROL:1) Cat. No. 1303 - Human Brain Tissue Lysate

psi-iconProperties
PURIFICATION:Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
CLONALITY:Polyclonal
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
CONCENTRATION:500 ug/mL
STORAGE CONDITIONS:Aliquot and store at -20˚C. Minimize freezing and thawing.

psi-iconAdditional Info
OFFICIAL SYMBOL:AR
ALTERNATE NAMES:AR, androgen receptor, dihydrotestosterone receptor, KD, AIS, TFM, DHTR, SBMA, NR3C4, SMAX1, HUMARA, HYSP1, androgen receptor, dihydrotestosterone receptor, RP11-383C12.1
ACCESSION NO.:NP_000035.2
PROTEIN GI NO.:21322252
GENE ID:367
psi-iconBackground and References
REFERENCES:1) Trapman J, Klaassen P, Kuiper GG, van der Korput JA, Faber PW, van Rooij HC, Geurts van Kessel A, Voorhorst MM, Mulder E, Brinkmann AO. Cloning, structure and expression of a cDNA encoding the human androgen receptor. Biochem Biophys Res Commun. 1988 May 31;153(1):241-8.

ANTIBODIES FOR RESEARCH USE ONLY.

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