AMPK alpha2 Antibody Cat. No.: 62-889

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Mouse, Rat
IMMUNOGEN: This AMPK alpha2 (PRKAA2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 453-483 amino acids from the C-terminal region of human AMPK alpha2 (PRKAA2).
TESTED APPLICATIONS: IHC-P, WB
APPLICATIONS: For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
PREDICTED MOLECULAR WEIGHT: 62 kDa

psi-iconProperties
PURIFICATION:This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
CLONALITY:Polyclonal
ISOTYPE:Rabbit Ig
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:Supplied in PBS with 0.09% (W/V) sodium azide.
CONCENTRATION:batch dependent
STORAGE CONDITIONS:Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:PRKAA2
ALTERNATE NAMES:5'-AMP-activated protein kinase catalytic subunit alpha-2, AMPK subunit alpha-2, Acetyl-CoA carboxylase kinase, ACACA kinase, Hydroxymethylglutaryl-CoA reductase kinase, HMGCR kinase, PRKAA2, AMPK, AMPK2
ACCESSION NO.:P54646
PROTEIN GI NO.:20178276
GENE ID:5563
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.
REFERENCES:1) Wyatt,C.N., J. Biol. Chem. 282 (11), 8092-8098 (2007)
2) Cheung,S.T., Neoplasia(9), 696-701 (2006)
3) Lee-Young,R.S., Am. J. Physiol. Endocrinol. Metab. 291 (3), E566-E573 (2006)
4) Gregory,S.G., Nature 441 (7091), 315-321 (2006)

ANTIBODIES FOR RESEARCH USE ONLY.

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