Specifications
HOST SPECIES: | Rabbit |
SPECIES REACTIVITY: |
Human, Mouse, Rat |
IMMUNOGEN: |
A synthetic peptide of human AMPK alpha 1 |
TESTED APPLICATIONS: |
IHC, WB |
APPLICATIONS: |
WB: ,1:500 - 1:2000
IHC: ,1:50 - 1:200 |
POSITIVE CONTROL: | 1) HeLa |
| 2) 293T |
| 3) MCF7 |
| 4) NIH/3T3 |
| 5) mouse brain |
PREDICTED MOLECULAR WEIGHT: |
Observed: 64kDa |
Properties
PURIFICATION: | Affinity purification |
CLONALITY: | Polyclonal |
ISOTYPE: | IgG |
CONJUGATE: | Unconjugated |
PHYSICAL STATE: | Liquid |
BUFFER: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
STORAGE CONDITIONS: | Store at -20˚C. Avoid freeze / thaw cycles. |
Additional Info
OFFICIAL SYMBOL: | PRKAA1 |
ALTERNATE NAMES: | PRKAA1, AMPK, AMPKa1, MGC33776, MGC57364 |
GENE ID: | 5562 |
USER NOTE: | Optimal dilutions for each application to be determined by the researcher. |
Background and References
BACKGROUND: | The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. |
ANTIBODIES FOR RESEARCH USE ONLY.
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