Specifications
| HOST SPECIES: | Goat |
| SPECIES REACTIVITY: |
Human, Mouse |
| IMMUNOGEN: |
Amphiphysin antibody was raised against a 12 amino acid synthetic peptide near the internal region of Amphiphysin. |
| TESTED APPLICATIONS: |
ELISA, IHC-P, WB |
| APPLICATIONS: |
ELISA: antibody detection limit dilution 1:16000. Western Blot: Approx 125kDa band observed in mouse brain lysates (calculated MW of 76.3kDa according to NP_001626.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Wigge et al, Mol Biol Cell. 1997 Immunohistochemistry: In paraffin embedded Human Cerebellum shows nuclear and fibrous staning. Recommended concentration, 2-4μg/ml. |
| SPECIFICITY: |
This antibody is expected to recognise both reported isoforms (NP_001626.1 and NP_647477.1). |
| POSITIVE CONTROL: | 1) Cat. No. 1403 - Mouse Brain Tissue Lysate |
Properties
| PURIFICATION: | Amphiphysin antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| CLONALITY: | Polyclonal |
| CONJUGATE: | Unconjugated |
| PHYSICAL STATE: | Liquid |
| BUFFER: | AmpH ipH ysin antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. |
| CONCENTRATION: | 500 ug/mL |
| STORAGE CONDITIONS: | Aliquot and store at -20˚C. Minimize freezing and thawing. |
Additional Info
| OFFICIAL SYMBOL: | AMPH |
| ALTERNATE NAMES: | AMPH, amphiphysin (Stiff-Man syndrome with breast cancer 128 kDa autoantigen), HGNC:471, AMPH1, amphiphysin, amphiphysin (Stiff-Mann syndrome with breast cancer 128kD autoantigen) |
| ACCESSION NO.: | NP_001626.1, NP_647477.1 |
| PROTEIN GI NO.: | 4502081 |
| GENE ID: | 273 |
Background and References
| REFERENCES: | 1) Sommer C, Weishaupt A, Brinkhoff J, Biko L, Wessig C, Gold R, Toyka KV. Paraneoplastic stiff-person syndrome: passive transfer to rats by means of IgG antibodies to amphiphysin. Lancet. 2005 Apr;365(9468):1406-11. |
ANTIBODIES FOR RESEARCH USE ONLY.
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