Amphiphysin Antibody Cat. No.: 45-006

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psi-iconSpecifications
HOST SPECIES:Goat
SPECIES REACTIVITY: Human, Mouse
HOMOLOGY: Expected Species Reactivity based on sequence homology: Rat, Dog
IMMUNOGEN: The immunogen for this antibody is: C-KRKDESRISKAE
TESTED APPLICATIONS: ELISA, IHC, WB
APPLICATIONS: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 125kDa band observed in mouse brain lysates (calculated MW of 76.3kDa according to NP_001626.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Wigge et al, Mol Biol Cell. 1997 Oct;8(10):2003-15.; PMID: 9348539). Recommended concentration: 0.1-0.5ug/ml. A minor band of unknown identity was also consistently observed at 45kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Cerebellum shows nuclear and fibrous staning. Recommended concentration, 2-4ug/ml.
SPECIFICITY: This antibody is expected to recognise both reported isoforms (NP_001626.1 and NP_647477.1).
POSITIVE CONTROL:1) Cat. No. 1403 - Mouse Brain Tissue Lysate

psi-iconProperties
PURIFICATION:Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
CLONALITY:Polyclonal
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
CONCENTRATION:500 ug/mL
STORAGE CONDITIONS:Aliquot and store at -20˚C. Minimize freezing and thawing.

psi-iconAdditional Info
OFFICIAL SYMBOL:AMPH
ALTERNATE NAMES:AMPH, amphiphysin, amphiphysin (Stiff-Man syndrome with breast cancer 128kDa autoantigen) , HGNC:471, AMPH1
ACCESSION NO.:NP_001626.1, NP_647477.1
PROTEIN GI NO.:4502081
GENE ID:273
psi-iconBackground and References
REFERENCES:1) Sommer C, Weishaupt A, Brinkhoff J, Biko L, Wessig C, Gold R, Toyka KV. Paraneoplastic stiff-person syndrome: passive transfer to rats by means of IgG antibodies to amphiphysin. Lancet. 2005 Apr;365(9468):1406-11.

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