Amphiphysin Antibody Cat. No.: 45-006

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psi-iconSpecifications
HOST SPECIES:Goat
SPECIES REACTIVITY: Human, Mouse
IMMUNOGEN: Amphiphysin antibody was raised against a 12 amino acid synthetic peptide near the internal region of Amphiphysin.
TESTED APPLICATIONS: ELISA, IHC-P, WB
APPLICATIONS: ELISA: antibody detection limit dilution 1:16000. Western Blot: Approx 125kDa band observed in mouse brain lysates (calculated MW of 76.3kDa according to NP_001626.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Wigge et al, Mol Biol Cell. 1997 Immunohistochemistry: In paraffin embedded Human Cerebellum shows nuclear and fibrous staning. Recommended concentration, 2-4μg/ml.
SPECIFICITY: This antibody is expected to recognise both reported isoforms (NP_001626.1 and NP_647477.1).
POSITIVE CONTROL:1) Cat. No. 1403 - Mouse Brain Tissue Lysate

psi-iconProperties
PURIFICATION:Amphiphysin antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
CLONALITY:Polyclonal
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:AmpH ipH ysin antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
CONCENTRATION:500 ug/mL
STORAGE CONDITIONS:Aliquot and store at -20˚C. Minimize freezing and thawing.

psi-iconAdditional Info
OFFICIAL SYMBOL:AMPH
ALTERNATE NAMES:AMPH, amphiphysin (Stiff-Man syndrome with breast cancer 128 kDa autoantigen), HGNC:471, AMPH1, amphiphysin, amphiphysin (Stiff-Mann syndrome with breast cancer 128kD autoantigen)
ACCESSION NO.:NP_001626.1, NP_647477.1
PROTEIN GI NO.:4502081
GENE ID:273
psi-iconBackground and References
REFERENCES:1) Sommer C, Weishaupt A, Brinkhoff J, Biko L, Wessig C, Gold R, Toyka KV. Paraneoplastic stiff-person syndrome: passive transfer to rats by means of IgG antibodies to amphiphysin. Lancet. 2005 Apr;365(9468):1406-11.

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