AMIGO1 Antibody Cat. No.: 7565

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: AMIGO1 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human AMIGO1.

The immunogen is located within the last 50 amino acids of AMIGO1.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: AMIGO1 antibody can be used for detection of AMIGO1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
SPECIFICITY: AMIGO1 antibody is human, mouse, and rat reactive. AMIGO1 antibody is predicted to not cross-react with AMIGO2 or AMIGO3.
POSITIVE CONTROL:1) Cat. No. 1201 - HeLa Cell Lysate
 2) Cat. No. 10-301 - Human Brain Tissue Slide
PREDICTED MOLECULAR WEIGHT: Predicted: 54 kDa

Observed: 55 kDa

psi-iconProperties
PURIFICATION:AMIGO1 antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:AMIGO1 antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:AMIGO1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:AMIGO1
ALTERNATE NAMES:AMIGO1 Antibody: ALI2, AMIGO, AMIGO-1, ALI2, KIAA1163, Amphoterin-induced protein 1
ACCESSION NO.:NP_065754
PROTEIN GI NO.:153791466
GENE ID:57463
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:The amphoterin-induced gene and ORF (AMIGO1) protein is a brain-enriched, glycosylated transmembrane immunoglobulin (Ig) superfamily protein with six extracellular leucine-rich repeats (LRRs) and one Ig-like domain. It and the related proteins AMIGO2 and AMIGO3 are thought to be cell adhesion molecules expressed on fiber tracts of neuronal tissues and participate in their formation (1). AMIGO1 has also been suggested to play important roles in dendritic outgrowth during development and could modulate the survival of developing and adult neurons (2).
REFERENCES:1) Kuja-Panula J, Kiiltomaki M, Yamashiro T, et al. AMIGO, a transmembrane protein implicated in axon tract development, defines a novel protein family with leucine-rich repeats. J. Cell Biol. 2003; 160:963-73.
2) Chen Y, Hor HH, and Tang BL. AMIGO is expressed in multiple brain cell types and may regulate dendritic growth and neuronal survival. J. Cell Physiol. 2012; 227:2217-29.

ANTIBODIES FOR RESEARCH USE ONLY.

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