Alzheimers Disease gamma-Secretase Detection Set Cat. No.: PSI-1820

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APPLICATIONS:These polyclonal antibodies can be used for detection of APH1, nicastrin, PEN2 and presenilin1 by immunoblot at 0.5 - 4 μg/mL, and Immunoflourescence.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
IMMUNOGEN:Rabbit polyclonal antibodies were raised against peptides corresponding to amino acid sequences from each of the corresponding proteins.
psi-iconSet Contents
Catalog Number 4001Catalog number 4001 — APH1 Antibody
Catalog Number 3983Catalog number 3983 — Nicastrin Antibody
Catalog Number 3979Catalog number 3979 — PEN2 Antibody
Catalog Number 4203Catalog number 4203 — Presenilin1 Antibody
BUFFER:PBS containing 0.02% sodium azide.
CONCENTRATION:Antibody 1 mg/mL
STORAGE CONDITIONS:Stable at 4˚C for three months, store at -20˚C for up to one year.
psi-iconAdditional Info
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
BACKGROUND:Accumulation of the amyloid-β peptide (Aβ) in the cerebral cortex is a critical event in the pathogenesis of Alzheimer’s disease. The β-amyloid protein precursor (APP) is cleaved by three enzymes (TACE, BACE/BACE2 and γ-secretase) at three distinct sites (α, β and γ respectively). The γ-secretase complex is a membrane-bound aspartyl protease that can cleave certain proteins at peptide bonds buried within the hydrophobic environment of the lipid bilayer and is composed of the proteins APH1, nicastrin, PEN2 and presenilin1. Its cleavage of APP results in either the non-toxic p3 (from the α and γ cleavage site) or the toxic Aβ (from the β and γ cleavage site). APH1 was initially identified as a component of the Notch pathway and exists in at least three distinct isoforms with APH1a as the principal isoform present in the γ-secretase complex. Mice deficient in this isoform, but not the other two, were lethal at E10.5, with impaired vascular and neural development observed. Besides acting as a critical component of the γ-secretase complex, nicastrin is also thought to be involved in cell proliferation and signaling, especially in regards to activation of Notch receptors as loss of nicastrin expression results in mouse embryonic lethality. Presenilin1 was initially identified a marker of susceptibility to early-onset Alzheimer’s disease.

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