| HOST SPECIES: | Chicken |
| SPECIES REACTIVITY: | Human, Mouse, Rat |
| HOMOLOGY: | Predicted species reactivity based on immunogen sequence: Bovine: (100%), Pig: (100%), Chicken: (100%) |
| IMMUNOGEN: | Tubulin antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human Tubulin. The immunogen is located within amino acids 140 - 190 of Alpha-tubulin. |
| TESTED APPLICATIONS: | ELISA, WB |
| APPLICATIONS: | Tubulin antibody can be used for detection of Tubulin by Western blot at 0.5 - 1 μg/mL. Antibody validated: Western Blot in rat samples. All other applications and species not yet tested. |
| POSITIVE CONTROL: | 1) Cat. No. 1463 - Rat Brain Tissue Lysate |
Specifications| PURIFICATION: | Alpha-tubulin Antibody is affinity chromatography purified via peptide column. |
| CLONALITY: | Polyclonal |
| ISOTYPE: | IgY |
| CONJUGATE: | Unconjugated |
| PHYSICAL STATE: | Liquid |
| BUFFER: | Alpha-tubulin Antibody is supplied in PBS containing 0.02% sodium azide. |
| CONCENTRATION: | 1 mg/mL |
| STORAGE CONDITIONS: | Alpha-tubulin antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| OFFICIAL SYMBOL: | TUBA3C |
| ALTERNATE NAMES: | Alpha-tubulin Antibody: TUBA2, bA408E5.3, TUBA2, TUBA3D, Alpha-tubulin 2 |
| ACCESSION NO.: | NP_005992 |
| PROTEIN GI NO.: | 17921993 |
| GENE ID: | 7278 |
| USER NOTE: | Optimal dilutions for each application to be determined by the researcher. |
| BACKGROUND: | Alpha-tubulin Antibody: Alpha-tubulin belongs to the tubulin superfamily, which is composed of six distinct families. Along with beta-tubulins, alpha-tubulins are the major components of microtubules. These microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Alpha- and beta-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha-tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Another post-translational modification of detyrosinated alpha-tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle. Like GAPDH and beta-Actin, this antibody makes an excellent loading control in immunoblots. |
| REFERENCES: | 1) McKean PG, Vaughan S, and Gull K. The extended tubulin family. J. Cell Sci.2001; 114:2723-33. | 2) Barra HA, Arce CA, and Argarana CE. Posttranslational tyrosination/detyrosination of tubulin. Mol. Neurobiol.1988; 2:133-53. | 3) Fukshima N, Furuta D, Hidaka Y, et al. Post-translational modifcations of tubulin in the nervous system. J. Neurochem.2009; 109:683-693. |
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