Alpha Fetoprotein Antibody [C3] Cat. No.: 33-066

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psi-iconSpecifications
HOST SPECIES:Mouse
SPECIES REACTIVITY: Dog, Human, Monkey, Pig
IMMUNOGEN: Alpha fetoprotein purified from serum of a hepatoma patient was used as the immunogen for this AFP antibody.
TESTED APPLICATIONS: Flow, IF, IHC, WB
APPLICATIONS: Flow Cytometry: 0.5-1 ug/million cells

IF: 0.5-1 ug/ml

IHC(FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)

Prediluted format : incubate for 30 min at RT (2)

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the AFP antibody to be titered up or down for optimal performance.

1. No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

SPECIFICITY: Does not react with cow, cat, mouse, rat.

psi-iconProperties
PURIFICATION:Protein G affinity chromatography
CLONALITY:Monoclonal
ISOTYPE:IgG2a, kappa
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:PBS with 0.1 mg/ml BSA and 0.05% sodium azide
CONCENTRATION:0.2 mg/mL
STORAGE CONDITIONS:Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.

psi-iconAdditional Info
OFFICIAL SYMBOL:AFP
ALTERNATE NAMES:AFP Antibody: FETA, HPAFP, Alpha-fetoprotein, Alpha-1-fetoprotein
GENE ID:174
USER NOTE:Optimal dilutions for each application to be determined by the researcher
psi-iconBackground and References
BACKGROUND:This antibody recognizes an oncofetal glycoprotein with a single chain of 70kDa, which is identified as Alpha fetoprotein (AFP). The AFP antibody is highly specific and shows no cross-reaction with other oncofetal antigens or serum albumin. Alpha fetoprotein is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. AFP antibody has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.

ANTIBODIES FOR RESEARCH USE ONLY.

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