| HOST SPECIES: | Rabbit |
| SPECIES REACTIVITY: | Human, Mouse, Rat |
| IMMUNOGEN: | Aldh5A1 antibody was raised against a 22 amino acid synthetic peptide near the carboxy terminus of the human Aldh5A1. The immunogen is located within the last 50 amino acids of Aldh5A1. |
| TESTED APPLICATIONS: | ELISA, WB |
| APPLICATIONS: | Aldh5A1 antibody can be used for detection of Aldh5A1 by Western blot at 0.25 - 0.5 μg/mL. Antibody validated: Western Blot in human samples. All other applications and species not yet tested. |
| POSITIVE CONTROL: | 1) Cat. No. 1304 - Human Liver Tissue Lysate |
| PREDICTED MOLECULAR WEIGHT: | Predicted: 60 kDa Observed: 60 kDa |
Specifications| PURIFICATION: | Aldh5A1 Antibody is affinity chromatography purified via peptide column. |
| CLONALITY: | Polyclonal |
| ISOTYPE: | IgG |
| CONJUGATE: | Unconjugated |
| PHYSICAL STATE: | Liquid |
| BUFFER: | Aldh5A1 Antibody is supplied in PBS containing 0.02% sodium azide. |
| CONCENTRATION: | 1 mg/mL |
| STORAGE CONDITIONS: | Aldh5A1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| OFFICIAL SYMBOL: | ALDH5A1 |
| ALTERNATE NAMES: | Aldh5A1 Antibody: SSDH, SSADH, Succinate-semialdehyde dehydrogenase, mitochondrial, Aldehyde dehydrogenase family 5 member A1 |
| ACCESSION NO.: | NP_733936 |
| PROTEIN GI NO.: | 25777721 |
| GENE ID: | 7915 |
| USER NOTE: | Optimal dilutions for each application to be determined by the researcher. |
| BACKGROUND: | Aldh5A1 Antibody: Aldh5A1 is a member of the aldehyde dehydrogenase superfamily, a group of NAD(P)(+)-dependent enzymes that catalyze the oxidation of a wide spectrum of aliphatic and aromatic aldehydes. Aldehyde dehydrogenase enzymes are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. Aldh5A1 is a mitochondrial NAD(+)-dependent succinic semialdehyde dehydrogenase. A deficiency of this enzyme, known as 4-hydroxybutyricaciduria, results in a disorder of the neurotransmitter 4-aminobutyric acid (GABA). Symptoms usually include static encephalopathy, associated with developmental delays, hypotonia, ataxia, speech defects, and seizures. At least two isoforms of Aldh5A1 are known to exist. |
| REFERENCES: | 1) Vasiliou V and Pappa A. Polymorphisms of human aldehyde dehydrogenases. Consequences for drug metabolism and disease. Pharmacology2000; 61:192-8. | 2) Hearl WG and Churchich JE. Interactions between4-aminobutyrate aminotransferase and succinic semialdehyde dehydrogenase, two mitochondrial enzymes. J. Biol. Chem.1984; 259:11459-63. | 3) Gibson KM, Sweetman L, Nyhan WL, et al. Succinic semialdehyde dehydrogenase deficiency: an inborn error of gamma-aminobutyric acid metabolism. Clin. Chim. Acta1983; 133:33-42. |
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