Albumin Antibody Cat. No.: 5159

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psi-iconSpecifications
HOST SPECIES:Chicken
SPECIES REACTIVITY: Human, Mouse, Rat
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Donkey: (85%), Horse: (85%), Pig: (85%), Rabbit: (85%), Chicken: (80%)
IMMUNOGEN: Albumin antibody was raised against a 13 amino acid synthetic peptide near the center of human Albumin.

The immunogen is located within amino acids 340 - 390 of Albumin.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: Albumin antibody can be used for detection of Albumin by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
POSITIVE CONTROL:1) Cat. No. 1404 - Mouse Liver Tissue Lysate
 2) Cat. No. 10-201 - Human Liver Tissue Slide
 3) Cat. No. 1304 – Human Liver Tissue Lysate
PREDICTED MOLECULAR WEIGHT: Predicted: 67 kDa

Observed: 57, 67 kDa

psi-iconProperties
PURIFICATION:Albumin Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgY
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:Albumin Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:Albumin antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:ALB
ALTERNATE NAMES:Albumin Antibody: PRO0883, PRO0903, PRO1341, Serum albumin
ACCESSION NO.:P02768
PROTEIN GI NO.:113576
GENE ID:213
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:Albumin Antibody: Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. It functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The resulting product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin. At least two isoforms of Albumin are known to exist.
REFERENCES:1) Rothschild MA, Oratz M, and Schreiber SS. Serum Albumin. Hepatology 1988; 8:385-401.

ANTIBODIES FOR RESEARCH USE ONLY.

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