AKT1S1 Antibody Cat. No.: 7391

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: AKT1S1 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human AKT1S1.

The immunogen is located within amino acids 190 - 240 of AKT1S1.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: AKT1S1 antibody can be used for detection of AKT1S1 by Western blot at 1 - 2 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
SPECIFICITY: AKT1S1 antibody is human, mouse and rat reactive. At least three isoforms of AKT1S1 are known to exist.
POSITIVE CONTROL:1) Cat. No. 1303 - Human Brain Tissue Lysate
PREDICTED MOLECULAR WEIGHT: Predicted: 30 kDa

Observed: 28 kDa

psi-iconProperties
PURIFICATION:AKT1S1 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:AKT1S1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:AKT1S1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:AKT1S1
ALTERNATE NAMES:AKT1S1 Antibody: Lobe, PRAS40, Proline-rich AKT1 substrate 1, 40 kDa proline-rich AKT substrate
ACCESSION NO.:NP_115751
PROTEIN GI NO.:400153980
GENE ID:84335
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:AKT1S1 Antibody: The Akt signaling pathway contributes to the regulation of apoptosis after a variety of cell death signals. AKT1S1, also known as PRAS40, is a proline-rich substrate of the kinase AKT1 and is thought to play a role in neuroprotection mediated by nerve growth factor (NGF) after transient focal cerebral ischemia (1). AKT1S1 is also a substrate and potential regulator of mammalian target of rapamycin (mTOR), a serine/threonine kinase that regulates cell growth and cell cycle, and a negative regulator of autophagy (2). Treatment with the insulin-like growth factor-1 (IGF1) can indusce the phosphorylation of AKT1S1 via the PI3K/AKT signaling pathway in PC12 cells (3).
REFERENCES:1) Saito A, Narasimhan P, Hayashi T, et al. Neuroprotective role of a proline-rich Akt substrate in apoptotic neuronal cell death after stroke: relationships with nerve growth factor. J. Neurosci. 2004; 24:1584-93.
2) Wiza C, Nascimento EB, and Ouwens DM. Role of PRAS40 in Akt and mTOR signaling in health and disease. Am. J. Physiol. Endocrinol. Metab. 2012; 302:E1453-60.
3) Wang H, Zhang Q, Zhang L, et al. Insulin-like growth factor-1 induces the phosphrylation of PRAS40 via the PI3K/Akt signaling pathway in PC12 cells. Neurosci. Lett. 516:105-9.

ANTIBODIES FOR RESEARCH USE ONLY.

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