AKT1 Monoclonal Antibody [A2D3] Cat. No.: 13-891

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: Recombinant protein of human AKT1
TESTED APPLICATIONS: IF, IHC, IP, WB
APPLICATIONS: WB: ,1:500 - 1:2000

IHC: ,1:50 - 1:200

IF: ,1:50 - 1:200

IP: ,1:20 - 1:50
POSITIVE CONTROL:1) MCF7
PREDICTED MOLECULAR WEIGHT: Observed: 56kDa

psi-iconProperties
PURIFICATION:Affinity purification
CLONALITY:Monoclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
STORAGE CONDITIONS:Store at -20˚C. Avoid freeze / thaw cycles.

psi-iconAdditional Info
OFFICIAL SYMBOL:AKT1
ALTERNATE NAMES:Akt1 Antibody: AKT, PKB, RAC, CWS6, PRKBA, PKB-ALPHA, RAC-ALPHA, Protein kinase B
GENE ID:207
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene.

ANTIBODIES FOR RESEARCH USE ONLY.

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