Akt (phospho Thr308) Antibody Cat. No.: 79-131

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Mouse, Rat
IMMUNOGEN: Akt (Phospho-Thr308) antibody was raised against a peptide sequence around phosphorylation site of threonine 308 (M-K-T (p) -F-C) derived from Human Akt.
TESTED APPLICATIONS: IF, IHC
APPLICATIONS: Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
SPECIFICITY: This antibody detects endogenous level of Akt only when phosphorylated at threonine 308.
PREDICTED MOLECULAR WEIGHT: 60 kDa

psi-iconProperties
PURIFICATION:Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
CLONALITY:Polyclonal
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:Store antibody at -20˚C for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:AKT1
ALTERNATE NAMES:AKT, PKB, RAC, CWS6, PRKBA, PKB-ALPHA, RAC-ALPHA, CFL, C-AKT, PKB-alpha, RAC-PK-alpha
ACCESSION NO.:NP_001014431.1
PROTEIN GI NO.:62241013
GENE ID:207
psi-iconBackground and References
BACKGROUND:General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.
REFERENCES:1) Tremblay F, et al. (2005) Diabetes; 54 (9): 2674-84. Xu BE, et al. (2005) J Biol Chem; 280 (40): 34218-23. Samuels Y, et al. (2005) Cancer Cell; 7 (6): 561-73. Di Maira G, et al. (2005) Cell Death Differ; 12 (6): 668-77.

ANTIBODIES FOR RESEARCH USE ONLY.

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