Akt (phospho Ser129) Antibody Cat. No.: 79-966

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: Akt (phospho Ser129) antibody was raised against a peptide sequence around phosphorylation site of serine129 (D-N-S (p)-G-A) derived from Human Akt .
TESTED APPLICATIONS: WB
APPLICATIONS: Western Blot: 1:500~1:1000
SPECIFICITY: The antibody detects endogenous level of Akt only when phosphorylated at serine 129.
PREDICTED MOLECULAR WEIGHT: 65 kDa

psi-iconProperties
PURIFICATION:Antibodies were purified by affinity-chromatography using epitope-specific peptide.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:Store antibody at -20˚C for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:AKT1
ALTERNATE NAMES:AKT, AKT1 kinase, kinase Akt1, RAC, RAC-PK-alpha
ACCESSION NO.:NP_001014431.1
PROTEIN GI NO.:62241013
GENE ID:207
psi-iconBackground and References
BACKGROUND:General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.
REFERENCES:1) Nguyen le XT, Mitchell BS (2013) Proc Natl Acad Sci U S A 110, 20681-6
2) Ponce DP, et al. (2011) Mol Cell Biochem 356, 127-32
3) Siddiqui-Jain A, et al. (2010) Cancer Res 70, 10288-98

ANTIBODIES FOR RESEARCH USE ONLY.

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