Aipl1 Antibody Cat. No.: 4865

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Bovine: (78%)
IMMUNOGEN: Aipl1 antibody was raised against a 18 amino acid synthetic peptide near the center of the human Aipl1.

The immunogen is located within amino acids 140 - 190 of Aipl1.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: Aipl1 antibody can be used for detection of Aipl1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
POSITIVE CONTROL:1) Cat. No. 1463 - Rat Brain Tissue Lysate
 2) Cat. No. 10-301 - Human Brain Tissue Slide

psi-iconProperties
PURIFICATION:Aipl1 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:Aipl1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:Aipl1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:AIPL1
ALTERNATE NAMES:Aipl1 Antibody: LCA4, AIPL2, Aryl-hydrocarbon-interacting protein-like 1
ACCESSION NO.:NP_055151
PROTEIN GI NO.:74272276
GENE ID:23746
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:Aipl1 Antibody: Aipl1 was initially identified as a protein implicated in Leber congenital amaurosis (LCA), an autosomal recessive disorder thought to be caused by the abnormal development of photoreceptors. Aipl1 is a tetratricopeptide repeat protein that is highly homologous to ARA9, a protein involved in the HSP90-mediated nuclear translocation and transactivation of the aryl hydrocarbon receptor. Aipl1 has also been found to function as part of a chaperone heterocomplex, interacting with Hsp90 and Hsp70. Aipl1 also associates with the cell cycle regulator NUB1. It is thought that Aipl1 cooperates with Hsp70 but not Hsp90 to suppress the formation of NUB1 inclusions, and these interactions are necessary in the normal photoreceptor maturation, as mutations that lead to LCA also compromise the interactions with the Hsp chaperones. At least three isoforms of Aipl1 are known to exist.
REFERENCES:1) Sohocki MM, Brown SJ, Sullivan LS, et al. Mutations in a new photoreceptor-pineal gene on 17p cause Leber congenital amaurosis. Nat. Genet.2000; 24:79-83.
2) Ma Q and Whitlock JP Jr. A novel cytoplasmic protein that interacts with the Ah receptor, contains tetratricopeptide repeat motifs, and augments the transcriptional response to 2,3,7,8-tetrachloro-dibenzo-p-dioxin. J. Biol. Chem.1997; 272:8878-84.
3) Hidalgo-de-Quintana J, Evans RJ, Cheetham ME, et al. The Leber congenital amaurosis protein aipl1 functions as part of a chaperone complex. Invest. Ophthalmol. Vis. Sci.2008; 49:2878-87.
4) Akey DT, Zhu X, Dyer M, et al. The inherited blindness associated protein Aipl1 interacts with the cell cycle regulator protein NUB1. Hum. Mol. Genet.2002; 11:2723-33.

ANTIBODIES FOR RESEARCH USE ONLY.

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