AIMP1 Antibody Cat. No.: 7513

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Chicken: (73%)
IMMUNOGEN: AIMP1 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human AIMP1.

The immunogen is located within amino acids 240 - 290 of AIMP1.
TESTED APPLICATIONS: ELISA, IHC-P, WB
APPLICATIONS: AIMP1 antibody can be used for detection of AIMP1 by Western blot at 1 μg/ml.

Antibody validated: Western Blot in human, mouse, and rat samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.
SPECIFICITY: AIMP1 antibody is human, mouse and rat reactive. At least two isoforms of AIMP1 are known to exist; this antibody will recognize both isoforms. AIMP1 antibody is predicted to not cross-react with AIMP2.
POSITIVE CONTROL:1) Cat. No. 1463 - Rat Brain Tissue Lysate
 2) Cat. No. 1303 - Human Brain Tissue Lysate
 3) Cat. No. 1403 - Mouse Brain Tissue Lysate
PREDICTED MOLECULAR WEIGHT: Predicted: 46 kDa

Observed: 36 kDa

psi-iconProperties
PURIFICATION:AIMP1 antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:AIMP1 antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:AIMP1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:AIMP1
ALTERNATE NAMES:AIMP1 Antibody: p43, HLD3, EMAP2, SCYE1, EMAPII, Aminoacyl tRNA synthase complex-interacting multifunctional protein 1, Multisynthase complex auxiliary component p43, EMAP-2
ACCESSION NO.:NP_001135888
PROTEIN GI NO.:215490011
GENE ID:9255
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:AIMP1 (Endothelial monocyte-activating polypeptide II, EMAP2) is a proinflammatory cytokine for monocytes and granulocytes (1). It is specifically induced by apoptosis and is involved in the control of angiogenesis, inflammation, and wound healing (1,2). AIMP1 was identified as one of three auxiliary factors of the mammalian aminoacyl tRNA synthetase (ARS) complex. It binds and facilitates the catalytic reaction of arginyl-tRNA synthetase (2,3). Recent studies show that CD23 plays an essential role in the AIMP1-induced immune response and might be a target in the treatment of inflammatory diseases (4).
REFERENCES:1) Park SG, Jung KH, Lee JS, et al. Precursor of pro-apoptotic cytokine modulates aminoacylation activity of tRNA synthetase. J. Biol. Chem. 1999; 274:16673–6.
2) Park SG, Shin H, Shin YK, et al. The novel cytokine p43 stimulates dermal fibroblast proliferation and wound repair. Am. J. Pathol. 2005; 166:387–98.
3) Quevillon S, Agou F, Robinson JC, et al. The p43 component of the mammalian multi-synthetase complex is likely to be the precursor of the endothelial monocyte-activating polypeptide II cytokine. J. Biol. Chem. 1997; 272:32573–9.
4) Kwon HS, Park MC, Kim DG, et al. Identification of CD23 as a functional receptor for the proinflammatory cytokine AIMP1/p43. J. Cell Sci. 2012; 125:4620-9.

ANTIBODIES FOR RESEARCH USE ONLY.

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