AIF Antibody Cat. No.: 2301

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: AIF antibody was raised against a 14 amino acid peptide near the carboxy terminus of human AIF.

The immunogen is located within the last 50 amino acids of AIF.
TESTED APPLICATIONS: ELISA, ICC, WB
APPLICATIONS: AIF antibody can be used for detection of AIF by Western blot at 0.5 - 2 μg/mL. A 67 kDa band should be detected. Antibody can also be used for immunocytochemistry starting at 5 μg/mL.

Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
POSITIVE CONTROL:1) Cat. No. 1204 - K562 Cell Lysate
 2) Cat. No. 1404 - Mouse Liver Tissue Lysate
 3) Cat. No. 1464 - Rat Liver Tissue Lysate
 4) Cat. No. 17-004 - K-562 Cell Slide
PREDICTED MOLECULAR WEIGHT: 67 kDa

psi-iconProperties
PURIFICATION:AIF Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:AIF Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:batch dependent
STORAGE CONDITIONS:AIF antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:CNKSR1
ALTERNATE NAMES:AIF Antibody: CNK, KSR, CNK1, Connector enhancer of kinase suppressor of ras 1, CNK homolog protein 1, Connector enhancer of KSR 1
ACCESSION NO.:O95381
PROTEIN GI NO.:50400606
GENE ID:10256
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:AIF Antibody: Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins. A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF). Like the critical molecules, cytochrome c and caspase-9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection. AIF is highly conserved between human and mouse and widely expressed.
REFERENCES:1) Zamzami N, Kroemer G. Condensed matter in cell death. Nature 1999;401:127-8
2) Susin SA, Lorenzo HK, Zamzami N, et al. Molecular characterization of mitochondrial apoptosis-inducing factor. Nature 1999;397:441-6 (WD0800)

ANTIBODIES FOR RESEARCH USE ONLY.

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