AFP Antibody Cat. No.: 5869

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Pig: (91%), Sheep: (83%), Bovine: (83%)
IMMUNOGEN: AFP antibody was raised against a 14 amino acid synthetic peptide near the center of human AFP.

The immunogen is located within amino acids 340 - 390 of AFP.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: AFP antibody can be used for detection of AFP by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested.

psi-iconProperties
PURIFICATION:AFP Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:AFP Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:AFP antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:AFP
ALTERNATE NAMES:AFP Antibody: FETA, HPAFP, Alpha-fetoprotein, Alpha-1-fetoprotein
ACCESSION NO.:NP_001125
PROTEIN GI NO.:4501989
GENE ID:174
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:AFP Antibody: Alpha-fetoprotein (AFP) is a major plasma protein produced by the yolk sac and the liver during fetal development. In adults, AFP expression is often associated with hepatoma or teratoma. However, hereditary persistance of AFP may also be found in individuals with no obvious pathology. AFP is thought to be the fetal counterpart of serum albumin, and the AFP and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. AFP is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. AFP levels in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.
REFERENCES:1) Lafuste P, Robert B, Mondon F, et al. Alpha-fetoprotein gene expression in early and full-term human trophoblast. Placenta2002; 23:600-12.
2) Walhof CM, Van Sonderen L, Voute PA, et al. Half-life of alpha-fetoprotein in patients with a teratoma, endodermal sinus tumor, or hepatoblastoma. Pediatr. Hematol. Oncol.1988; 5:217-27.
3) Nishio H, Heiskanen M, Palotie A, et al. Tandem arrangement of the human serum albumin multigene family in the sub-centromeric region of 4q: evolution and chromosomal direction of transcription. J. Mol. Biol.1996; 259:113-9.
4) Deutsch HF. Chemistry and biology of alpha-fetoprotein. Adv. Cancer Res.1991; 56:253-312

ANTIBODIES FOR RESEARCH USE ONLY.

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