| HOST SPECIES: | Rabbit |
| SPECIES REACTIVITY: | Human, Mouse, Rat |
| HOMOLOGY: | Predicted species reactivity based on immunogen sequence: Bovine: (100%) |
| IMMUNOGEN: | ADRM1 antibody was raised against a 19 amino acid peptide near the center of human ADRM1. The immunogen is located within amino acids 210 - 260 of ADRM1. |
| TESTED APPLICATIONS: | ELISA, IHC-P, WB |
| APPLICATIONS: | ADRM1 antibody can be used for detection of ADRM1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. Antibody validated: Western Blot in mouse samples and Immunohistochemistry in human samples. All other applications and species not yet tested. |
| SPECIFICITY: | ADRM1 antibody is human, mouse and rat reactive. At least two isoforms of ADRM1 are know to exist; this antibody will detect both isoforms. |
| POSITIVE CONTROL: | 1) Cat. No. 1287 - EL4 Cell Lysate |
| 2) Cat. No. 10-901 - Human Spleen Tissue Slide | |
| PREDICTED MOLECULAR WEIGHT: | Predicted: 45 kDa Observed: 48 kDa |
Specifications| PURIFICATION: | ADRM1 antibody is affinity chromatography purified via peptide column. |
| CLONALITY: | Polyclonal |
| ISOTYPE: | IgG |
| CONJUGATE: | Unconjugated |
| PHYSICAL STATE: | Liquid |
| BUFFER: | ADRM1 antibody is supplied in PBS containing 0.02% sodium azide. |
| CONCENTRATION: | 1 mg/mL |
| STORAGE CONDITIONS: | ADRM1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| OFFICIAL SYMBOL: | ADRM1 |
| ALTERNATE NAMES: | Adhesion regulating molecule 1, ARM1, Gp110, hRpn13, Proteasomal ubiquitin receptor ADRM1, Proteasome regulatory particle non-ATPase 13, Rpn13 |
| ACCESSION NO.: | NP_783163 |
| PROTEIN GI NO.: | 28373194 |
| GENE ID: | 11047 |
| USER NOTE: | Optimal dilutions for each application to be determined by the researcher. |
| BACKGROUND: | The adhesion-regulating molecule 1 (ADRM1) is thought to be involved in protein recruitment and cell adhesion and functions as a proteasomal ubiquitin receptor (1). ADRM1 promotes the activity of UCH-L5 and plays a role in lymphocyte homing. The carboxy-terminal domain of mammalian ADRM1 serves to bind and enhance the isopeptidase activity of UCHL5/UCH37, perhaps serving as a mechanism to accelerate ubiquitin chain disassembly (2,3). ADRM1 expression is induced by IFN-gamma in some cancer cell lines and its expression is upregulated in other metastatic cells, suggesting a role in carcinogenesis (4,5). |
| REFERENCES: | 1) Husnjak K, Elsasser S, Zhang N, et al. Proteasome subunit Rpn13 is a novel ubiquitin receptor. Nature 2008; 453:481-8. | 2) Yao T, Song L, Xu W, et al. Proteasome recruitment and activation of the Uch37 deubiquitinating enzyme by Adrm1. Nat. Cell Biol. 2006; 8:994-1002. | 3) Qiu XB, Ouyang SY, Li CJ, et al. hRpn13/ADRM1/GP110 is a novel proteasome subunit that binds the deubiquitinating enzyme, UCH37. EMBO J. 2006; 25:5742-53. | 4) Al-Shami A, Jhaver KG, Vogel P, et al. Regulators of the proteasome pathway, Uch37 and Rpn13, play distinct roles in mouse development. PLoS One 2010; 5:e13654. |
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