ADORA2B Antibody Cat. No.: 8111

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Rabbit: (80%)
IMMUNOGEN: ADORA2B antibody was raised against a 19 amino acid peptide near the carboxy terminus of human ADORA2B.

The immunogen is located within the last 50 amino acids of ADORA2B.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: ADORA2B antibody can be used for detection of ADORA2B by Western blot at 0.25 – 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in mouse samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
SPECIFICITY: ADORA2B antibody is human, mouse and rat reactive. At least two isoforms of ADORA2B are known to exist; this antibody will detect both isoforms. ADORA2B antibody is predicted to not cross-react with ADORA2A.
POSITIVE CONTROL:1) Cat. No. 1405 - Mouse Kidney Tissue Lysate
 2) Cat. No. 1411 - Mouse Colon Tissue Lysate
PREDICTED MOLECULAR WEIGHT: Predicted: 37 kDa

Observed: 41 kDa

psi-iconProperties
PURIFICATION:ADORA2B antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:ADORA2B antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:ADORA2B antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:ADORA2B
ALTERNATE NAMES:Adenosine receptor A2b, ADORA2
ACCESSION NO.:NP_000667
PROTEIN GI NO.:4501951
GENE ID:136
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:The adenosine receptor ADORA2B is a member of the G protein-coupled receptor superfamily and is an integral membrane protein that stimulates adenylate cyclase activity in the presence of adenosine (1,2). Extracellular adenosine triggers a potent anti-inflammatory response that is mediated in part by ADORA2B including the stimulation of IL-10 production (3-5). Activation of ADORA2B can also enhance the abundance of regulatory T cells (Tregs), a class of cells that work to constrain inflammation (6).
REFERENCES:1) Pierce KD, Furlong TJ, Selbie LA, et al. Molecular cloning and expression of an adenosine A2b receptor from human brain. Biochem. Biophys. Res. Commun. 1992; 187:86-93.
2) Thimm D, Schiedel AC, Sherbiny FF, et al. Ligand-specific binding and activation of the human adenosine A(2B) receptor. Biochem. 2013; 52:726-40.
3) Blackburn MR, Vance CO, Morschl E, et al. Adenosine receptors and inflammation. Handb. Exp. Pharmacol. 2009; 215-69.
4) Grenz A, Homann D, Eltzschig HK. Extracellular adenosine: a safety signal that dampens hypoxia-induced inflammation during ischemia. Antioxid. Redox Signal. 2011; 15:2221-34.

ANTIBODIES FOR RESEARCH USE ONLY.

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