There is an ever-increasing need for peptides in biological and drug discovery research since peptides have a wide range of applications. They regulate most physiological processes, acting at some sites as endocrine or paracrine signals and at others as neurotransmitters or growth factors. Typically, peptides are used to develop new immunogens, hormones, and vaccines. Peptides that bind to the target antibody but simultaneously prevent nonspecific antibody binding are described as blocking peptides. By resembling the epitope, the antibody treats these peptides as actual epitopes, thus they are able to carry out this unique function. Organized by source species such as chicken, dog, drosophila, and human, here is an example of the high quality blocking peptides available through ProSci which include easy-to-follow protocol instructions. Our experience and extensive catalogue positions ProSci as the leading provider of blocking peptides.


Derived from Drosophila, E2F1 peptide is used for blocking the activity of E2F1 antibody. The E2F1 antibody ships at 0.5 mg/mL and the STK36 Peptide ships as a 100 ug pellet. When peptide is reconstituted in 200 uL water, the concentration will be 0.5 mg/mL, identical to that of the antibody. The best starting ratio would be 1:1 (which means molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volume of peptide and antibody at the required dilution and leave at ambient temperature for 20 minutes. Then incubate and develop in parallel two blots: one with the antibody and one with the antibody which has been pre-absorbed with the provided peptide.

With a catalogue of nearly 1600 research peptides for sale, and over 17 years of experience, ProSci is the leading supplier of 0.1mg blocking peptides. You can refine your search by source species including human, mouse, chicken and drosophila to find the blocking peptide that best meets your needs. If you are looking to buy peptides online, ProSci is the ideal choice!

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