ADAM17 Antibody Cat. No.: 45-002

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psi-iconSpecifications
HOST SPECIES:Goat
SPECIES REACTIVITY: Human
HOMOLOGY: Expected Species Reactivity based on sequence homology: Mouse, Rat
IMMUNOGEN: The immunogen for this antibody is: LQRQNRVDSKETEC
TESTED APPLICATIONS: ELISA, IHC, WB
APPLICATIONS: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 100kDa band observed in Human Testis lysates and approx 125kDa in lysates of cell lines Daudi, HeLa and Jurkat (calculated MW of 93.0kDa according to NP_003174.3). The observed molecular weights correspond to earlier findings in literature with different antibodies (Le Gall et al, J Cell Sci. 2010 Nov 15;123(Pt22):3913-22; PMID: 20980382). Recommended concentration: 0.1-0.3ug/ml. Primary incubated for 1 hour. Preliminary testing was unsuccessful on Mouse and Rat for this particular batch.Immunohistochemistry:Paraffin embedded Human Skin and Thyroid. Recommended concentration: 3.75ug/ml.
SPECIFICITY: This antibody is expected to recognise isoform 1 (represented by NP_003174.3) and not isoform 2 (represented by NP_068604).
POSITIVE CONTROL:1) Cat. No. 1201 - HeLa Cell Lysate

psi-iconProperties
PURIFICATION:Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
CLONALITY:Polyclonal
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
CONCENTRATION:500 ug/mL
STORAGE CONDITIONS:Aliquot and store at -20˚C. Minimize freezing and thawing.

psi-iconAdditional Info
OFFICIAL SYMBOL:ADAM17
ALTERNATE NAMES:ADAM17, TACE, a disintegrin and metalloproteinase domain 17 (tumor necrosis factor, alpha, converting enzyme), CSVP, cSVP, CD156b, CD156b, MGC71942, TACE, cSVPADAM metallopeptidase domain 17, TNF-alpha converting enzyme, a disintegrin and metalloproteinase domain 17, a disintegrin and metalloproteinase domain 17 (tumor necrosis factor, alpha, converting enzyme), snake venom-like protease, ADAM metallopeptidase domain 17, ADAM18, CD156B, ADAM metallopeptidase domain 18, snake venom-like protease, tumor necrosis factor, alpha, converting enzyme
ACCESSION NO.:NP_003174.3
PROTEIN GI NO.:73747889
GENE ID:6868
psi-iconBackground and References
REFERENCES:1) Black RA, Rauch CT, Kozlosky CJ, Peschon JJ, Slack JL, Wolfson MF, Castner BJ, Stocking KL, Reddy P, Srinivasan S, Nelson N, Boiani N, Schooley KA, Gerhart M, Davis R, Fitzner JN, Johnson RS, Paxton RJ, March CJ, Cerretti DP A metalloproteinase disintegrin that releases tumour-necrosis factor-alpha from cells. Nature. 1997 Feb 20;385(6618):729-33

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