Acetyl-Histone H4-K5 pAb Cat. No.: 15-941

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: A synthetic acetylated peptide around K5 of human Histone H4 (NP_001029249.1).
TESTED APPLICATIONS: ChIP, IF, IHC, WB
APPLICATIONS: WB: ,1:500 - 1:2000

IHC: ,1:50 - 1:200

IF: ,1:50 - 1:200

ChIP: ,1:20 - 1:100
POSITIVE CONTROL:1) HeLa
 2) C6
PREDICTED MOLECULAR WEIGHT: Observed: 11kDa

psi-iconProperties
PURIFICATION:Affinity purification
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
STORAGE CONDITIONS:Store at -20˚C. Avoid freeze / thaw cycles.

psi-iconAdditional Info
OFFICIAL SYMBOL:H4K5AC
ALTERNATE NAMES:H4/A, H4FA, HIST1H4B, H4/I, H4FI, HIST1H4C, H4/G, H4FG, HIST1H4D, H4/B, H4FB, HIST1H4E, H4/J, H4FJ, HIST1H4F, H4/C, H4FC, HIST1H4H, H4/H, H4FH, HIST1H4I, H4/M, H4FM, HIST1H4J, H4/E, H4FE, HIST1H4K, H4/D, H4FD, HIST1H4L, H4/K, H4FK, HIST2H4A, H4/N, H4F2, H4FN, HIST2H4, HIST2H4B, H4/O, H4FO, HIST4H4, Histone H4H4FA
GENE ID:8370
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.

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