ACE2 Antibody Cat. No.: 3215

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Bovine: (94%)
IMMUNOGEN: Anti-ACE2 antibody (3215) was raised against a peptide corresponding to 17 amino acids near the center of human ACE2.

The immunogen is located within amino acids 180 - 230 of ACE2.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: WB: 1-2 μg/mL; IHC: 2 μg/mL; IF: 20 μg/mL.

Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human samples; Immunofluorescence in human and rat samples. All other applications and species not yet tested.
SPECIFICITY: Anti-ACE2 has no cross response to ACE1.
POSITIVE CONTROL:1) Cat. No. 1305 - Human Kidney Tissue Lysate
 2) Cat. No. 10-401 - Human Kidney Tissue Slide
 3) Cat. No. 1306 - Human Spleen Tissue Lysate
 4) Cat. No. 1313 - Human Testis Tissue Lysate
PREDICTED MOLECULAR WEIGHT: Predicted: 93kD

Observed: 130 kD (7 N-linked glycosylation)
psi-iconAdvanced Validation
VALIDATION:

Independent Antibody Validation in Cell lines (Figure 1) shows similar ACE2 expression profile in human cell lines detected by three independent anti-ACE2 antibodies that recognize different epitopes, 3215 against central domain, 3217 against C-terminus domain and 3227 against N-terminus domain.  ACE2 proteins are detected in the most tested tissues at different expression levels by three independent antibodies.

ISOFORMS: Human ACE2 has 2 isoforms, including isoform 1 (805aa, 93kD) and isoform 2 (555aa, 64kD). Mouse ACE2 also has 2 isoforms, including isoform 1 (805aa, 92kD) and isoform 2 (353aa, 40kD). Rat ACE2 has one isoform (805aa, 93kD). 3215 can detect human, mouse and rat.

psi-iconProperties
PURIFICATION:ACE2 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:ACE2 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:ACE2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:ACE2
ALTERNATE NAMES:ACE2 Antibody: ACEH, Angiotensin-converting enzyme 2, ACE-related carboxypeptidase, ACEH, SARS-CoV receptor, SARS-CoV-2 receptor
ACCESSION NO.:NP_068576
PROTEIN GI NO.:11225609
GENE ID:59272
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:

ACE2 Antibody: Angiotensin-converting enzyme 2 (ACE2) plays a central role in vascular, renal, and myocardial physiology. In contrast to its homolog ACE, ACE2 expression is restricted to heart, kidney, and testis. Recently. ACE2 has also been shown to be a functional receptor of the SARS coronavirus. Homology modeling shows 2019-nCoV has a similar receptor-binding domain structure as SARS-CoV, which suggests COVID-19 (2019-nCoV) may use ACE2 as a receptor in humans for infection. The normal function of ACE2 is to convert the inactive vasoconstrictor angiotensin I (AngI) to Ang1-9 and the active form AngII to Ang1-7, unlike ACE, which converts AngI to AngII. While the role of these vasoactive peptides is not well understood, lack of ACE2 expression in ace2-/ace2- mice leads to severely reduced cardiac contractility, indicating its importance in regulating heart function.

REFERENCES:1) Donoghue et al. Circ. Res. 2000;87:1-9.
2) Tipnis et al. J Biol. Chem. 2000;275:33238-43.
3) Li et al. Nature 2003;426:450-4.
4) Lu et al. The Lancet 2020 (published online).
5) Crackower et al. Nature 2002;417:822-8.

ANTIBODIES FOR RESEARCH USE ONLY.

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