AATF Antibody Cat. No.: 27-426

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human
IMMUNOGEN: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human AATF.
TESTED APPLICATIONS: ELISA, IHC, WB
APPLICATIONS: AATF antibody can be used for detection of AATF by ELISA at 1:12500. AATF antibody can be used for detection of AATF by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
POSITIVE CONTROL:1) Cat. No. XBL-10408 - Fetal Kidney Tissue Lysate
PREDICTED MOLECULAR WEIGHT: 63 kDa

psi-iconProperties
PURIFICATION:Antibody is purified by peptide affinity chromatography method.
CLONALITY:Polyclonal
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
CONCENTRATION:batch dependent
STORAGE CONDITIONS:For short periods of storage (days) store at 4˚C. For longer periods of storage, store AATF antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.

psi-iconAdditional Info
OFFICIAL SYMBOL:AATF
ALTERNATE NAMES:AATF, DED, BFR2, CHE1, CHE-1
ACCESSION NO.:NP_036270
PROTEIN GI NO.:7657013
GENE ID:26574
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:The AATF gene encodes a protein that was identified on the basis of its interaction with MAP3K12/DLK, a protein kinase known to be involved in the induction of cell apoptosis. This gene product contains a leucine zipper, which is a characteristic motif of transcription factors, and was shown to exhibit strong transactivation activity when fused to Gal4 DNA binding domain. Overexpression of this gene interfered with MAP3K12 induced apoptosis.
REFERENCES:1) Burgdorf, S., et al., (2004) J. Biol. Chem. 279 (17), 17524-17534.

ANTIBODIES FOR RESEARCH USE ONLY.

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