Datasheet

A-RAF Antibody
CATALOG NUMBER: 63-215

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human
TESTED APPLICATIONS:IHC-P, WB
APPLICATIONS:For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
PREDICTED MOLECULAR WEIGHT:68 kDa
IMMUNOGEN:This A-RAF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 256-286 amino acids from the Central region of human A-RAF.
HOST SPECIES:Rabbit

Properties

PURIFICATION:This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
PHYSICAL STATE:Liquid
BUFFER:Supplied in PBS with 0.09% (W/V) sodium azide.
CONCENTRATION:2 mg/ml
STORAGE CONDITIONS:Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:Rabbit Ig
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:Serine/threonine-protein kinase A-Raf, Proto-oncogene A-Raf, Proto-oncogene A-Raf-1, Proto-oncogene Pks, ARAF, ARAF1, PKS, PKS2
ACCESSION NO.:P10398
PROTEIN GI NO.:1730068
OFFICIAL SYMBOL:ARAF
GENE ID:369

Background

BACKGROUND:Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.
REFERENCES: 1) Wu, X., et al., J. Biol. Chem. 271(6):3265-3271 (1996).
2) Lee, J.E., et al., Genomics 20(1):43-55 (1994).
3) Popescu, N.C., et al., Oncogene 4(4):517-519 (1989).
4) Beck, T.W., et al., Nucleic Acids Res. 15(2):595-609 (1987).

For Research Use Only