5'-Nucleotidase Recombinant Protein Cat. No.: 91-402

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psi-iconSpecifications
SPECIES:Human
SOURCE SPECIES:Human Cells
SEQUENCE:Trp27-Lys547
FUSION TAG:C-6 His tag
TESTED APPLICATIONS:
APPLICATIONS:This recombinant protein can be used for biological assays. For research use only.
psi-iconProperties
PURITY:Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin level less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.
PREDICTED MOLECULAR WEIGHT:58.76 kD
PHYSICAL STATE:Liquid
BUFFER:Supplied as a 0.2 um filtered solution of 20mM TrisHCl, 120mM NaCl,4mM CaCl2, 20% Glycerol, pH 7.5. It is not recommended to reconstitute to a concentration less than 100 ug/ml.
STORAGE CONDITIONS:Store at -20˚C, stable for 6 months after receipt.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
psi-iconAdditional Info
ALTERNATE NAMES:5'-Nucleotidase, 5'-NT, Ecto-5'-Nucleotidase, CD73, NT5E, NT5, NTE
ACCESSION NO.:P21589
PROTEIN GI NO.:578877726
OFFICIAL SYMBOL:NT5E
GENE ID:4907
psi-iconBackground and References
BACKGROUND:CD73 is a glycosyl phosphatidylinositol (GPI) anchored membrane protein that belongs to the 5'-nucleotidase family. CD73 is an ecto 5'Nucleotidase expressed by most cell types. CD73 hydrolyzes extracellular nucleotides into membrane permeable nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury. CD73 is a lymphocyte maturation marker that has functions independent of its catalytic activity. CD73 is also a regulator of leukocyte extravasation, a function that requires its 5'Nucleotidase activity. Defects in NT5E are the cause of calcification of joints and arteries (CAJA). The recombinant CD73 lacking GPI anchor is secreted as a monomer.

FOR RESEARCH USE ONLY.

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