4E-T Antibody Cat. No.: 45-177

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psi-iconSpecifications

HOST SPECIES:Goat
SPECIES REACTIVITY: Human
IMMUNOGEN: 4E-T antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of 4E-T.
TESTED APPLICATIONS: ELISA, IHC-P, WB
APPLICATIONS: ELISA: antibody detection limit dilution 1:32000. Western Blot: Approx 140-150kDa band observed in 293 lysates (predicted size of approx. 108kDa according to NP_062817.1, however our observation agrees with that of Dostie et al (see below) ). Recommended for use at 0.25-0.5μg/ml Immunohistochemistry: In paraffin embedded Human Pancreas shows strong staining of cytoplasm in acinar cells-. Recommended concentration, 5-10μg/ml.
SPECIFICITY: This antibody is expected to recognise isoform 1 (NP_062817.2) and isoform b (NP_001157974.1). Reported variants represent identical protein (NP_062817.2; NP_001157973.1).
POSITIVE CONTROL:1) Cat. No. 1210 - 293 Cell Lysate

psi-iconProperties

PURIFICATION:4E-T antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
CLONALITY:Polyclonal
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:4E-T antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
CONCENTRATION:500 ug/mL
STORAGE CONDITIONS:Aliquot and store at -20˚C. Minimize freezing and thawing.

psi-iconAdditional Info

OFFICIAL SYMBOL:EIF4ENIF1
ALTERNATE NAMES:EIF4ENIF1, 4E-T, Clast4, FLJ21601, 2610509L04Rik, eukaryotic translation initiation factor 4E nuclear import factor 1, eIF4E-transporter
ACCESSION NO.:NP_062817.1
PROTEIN GI NO.:9789865
GENE ID:56478

psi-iconBackground and References

REFERENCES:1) Dostie J, Ferraiuolo M, Pause A, Adam SA, Sonenberg N. A novel shuttling protein, 4E-T, mediates the nuclear import of the mRNA 5' cap-binding protein, eIF4E. EMBO J. 2000 Jun 15;19(12):3142-56.

ANTIBODIES FOR RESEARCH USE ONLY.

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