4E-BP1 Antibody [4B6G10] Cat. No.: 32-102

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psi-iconSpecifications
HOST SPECIES:Mouse
SPECIES REACTIVITY: Human
IMMUNOGEN: Ni-NTA purified truncated recombinant 4E-BP1 expressed in E. Coli strain BL21 (DE3).
TESTED APPLICATIONS: ELISA, WB
APPLICATIONS: Western Blot:1:500 - 1:1,000
IHC(P):1:500 - 1:1,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
POSITIVE CONTROL:1) Cat. No. 1202 - A431 Cell Lysate

psi-iconProperties
CLONALITY:Monoclonal
ISOTYPE:IgG1
CONJUGATE:Unconjugated
BUFFER:Ascitic fluid containing 0.03% sodium azide.
STORAGE CONDITIONS:4E-BP1 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:EIF4EBP1
ALTERNATE NAMES:Phosphorylated heat- and acid-stable protein regulated by insulin 1, 4E-BP1, BP-1, 4EBP1, PHAS-I
ACCESSION NO.:Q13541
PROTEIN GI NO.:34921508
GENE ID:1978
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:4E-BP1(eukaryotic translation Initiation Factor 4E Binding Protein 1),also called ELF4EBP1/BP-1/PHAS-I ,which is located on chromosome 8p12, with 118-amino acid protein (about 13 kDa). Binding of eIF4EBP1 to eIF4E is reversible and is dependent on the phosphorylation status of eIF4EBP1. Non phosphorylated eIF4EBP1 will bind strongly to eIF4E while(24 kDa), the phosphorylated form will not. Akt, TOR, MAP kinase, S6 kinase, and Cdc2 are known kinases capable of inactivating eIF4EBP1 binding to eIF4E by phosphorylating either threonines 35, 45, 69 or serine 64. Although, not all phosphorylation events equally block the eIF4EBP1-eIF4E interaction.
REFERENCES:1) Pause, A. et al. 1994.Nature. 371:762–767.
2) Fadden, P. et al. 1997. J. Biol. Chem. 272:10240–10247.

ANTIBODIES FOR RESEARCH USE ONLY.

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