4E-BP1 Antibody Cat. No.: 3513

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Rat: (100%), Bovine: (92%)
IMMUNOGEN: 4E-BP1 antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human 4E-BP1.

The immunogen is located within the last 50 amino acids of 4E-BP1.
TESTED APPLICATIONS: ELISA, ICC, IF, WB
APPLICATIONS: 4E-BP1 antibody can be used for the detection of 4E-BP1 by Western blot at 2.5 - 10 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
POSITIVE CONTROL:1) Cat. No. 1212 - 3T3 Cell Lysate
 2) Cat. No. 17-201 - 3T3/BALB Cell Slide

psi-iconProperties
PURIFICATION:4E-BP1 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:4E-BP1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:4E-BP1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:EIF4EBP1
ALTERNATE NAMES:4E-BP1 Antibody: BP-1, 4EBP1, 4E-BP1, PHAS-I, Eukaryotic translation initiation factor 4E-binding protein 1, Phosphorylated heat- and acid-stable protein regulated by insulin 1
ACCESSION NO.:NP_004086
PROTEIN GI NO.:4758258
GENE ID:1978
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:4E-BP1 Antibody: The translation of mRNA in eukaryotic cells is regulated by the presence of amino acids through multiple mechanisms. One such mechanism involves the evolutionarily conserved serine/threonine kinase TOR (Target of rapamycin, also known as mTOR), which regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. One downstream target of TOR is the eukaryotic initiation factor 4E binding protein 1 (4E-BP1) whose phosphorylation prevents its association with eIF4E, preferentially stimulating translation of mRNAs containing long, highly structured 5'-UTRs. Rapamycin inhibits TOR resulting in reduced cell growth and reduced rates of cell cycle and cell proliferationl, at least in part by inhibiting the activity of TOR towards 4E-BP1.
REFERENCES:1) Shah OJ, Anthony JC, Kimball SR, et al. 4E-BP1 and S6K1: translational integration sites for nutritional and hormonal information in muscle. Am. J. Physiol. Endocrinol. Metab. 2000; 279:E715-29.
2) Shamji AF, Ngheim P, and Schreiber SL. Integration of growth factor and nutrient signaling: implications for cancer biology. Mol. Cell 2003; 12:271-80.

ANTIBODIES FOR RESEARCH USE ONLY.

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