27Kip1 Antibody Cat. No.: 62-207

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Hamster
IMMUNOGEN: This 27Kip1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 165-194 amino acids from the C-terminal region of human 27Kip1.
TESTED APPLICATIONS: IF, WB
APPLICATIONS: For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50
PREDICTED MOLECULAR WEIGHT: 22 kDa

psi-iconProperties
PURIFICATION:This antibody is purified through a protein A column, followed by peptide affinity purification.
CLONALITY:Polyclonal
ISOTYPE:Rabbit Ig
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:Supplied in PBS with 0.09% (W/V) sodium azide.
CONCENTRATION:batch dependent
STORAGE CONDITIONS:Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:CDKN1B
ALTERNATE NAMES:Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1, CDKN1B, KIP1
ACCESSION NO.:P46527
GENE ID:1027
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:p27Kip1 is a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.
REFERENCES:1) Kawamata, N., et al., Eur. J. Haematol. 74(5):424-429 (2005).
2) Andreu, E.J., et al., Cancer Res. 65(8):3264-3272 (2005).
3) Wingate, H., et al., J. Biol. Chem. 280(15):15148-15157 (2005).
4) Wang, C., et al., J. Biol. Chem. 280(13):12339-12343 (2005).

ANTIBODIES FOR RESEARCH USE ONLY.

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