14-3-3 sigma Antibody Cat. No.: 45-174

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psi-iconSpecifications
HOST SPECIES:Goat
SPECIES REACTIVITY: Human, Mouse, Rat
HOMOLOGY: Expected Species Reactivity based on sequence homology: Dog, Pig
IMMUNOGEN: The immunogen for this antibody is: C-DLHTLSEDSYKDST
TESTED APPLICATIONS: ELISA, IHC, WB
APPLICATIONS: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 26-28kDa band observed in Human, Mouse and Rat Skin lysates and in lysates of cell lines A431 and U2OS (calculated MW of 27.8kDa according to Human NP_006133.1, Mouse NP_061224.2 and Rat XP_003750096.1). Recommended concentration: 0.03-0.1ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:In paraffin embedded Human Skin shows strong nuclear staining in selected cells of the basal layer. Recommended concentration, 2-4ug/ml.
POSITIVE CONTROL:1) Cat. No. 1376 - Human Skin Tissue Lysate

psi-iconProperties
PURIFICATION:Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
CLONALITY:Polyclonal
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
CONCENTRATION:500 ug/mL
STORAGE CONDITIONS:Aliquot and store at -20˚C. Minimize freezing and thawing.

psi-iconAdditional Info
OFFICIAL SYMBOL:SFN
ALTERNATE NAMES:SFN, 14-3-3sigma, stratifin, HGNC:10773, YWHAS
ACCESSION NO.:NP_006133.1
PROTEIN GI NO.:5454052
GENE ID:2810
psi-iconBackground and References
REFERENCES:1) Lodygin D, Hermeking H. The role of epigenetic inactivation of 14-3-3sigma in human cancer. Cell Res. 2005 Apr;15(4):237-46. Review.

ANTIBODIES FOR RESEARCH USE ONLY.

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