14-3-3 sigma Antibody Cat. No.: 45-174

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psi-iconSpecifications

HOST SPECIES:Goat
SPECIES REACTIVITY: Human
IMMUNOGEN: 14-3-3 sigma antibody was raised against a 14 amino acid synthetic peptide near the internal region of 14-3-3 sigma.
TESTED APPLICATIONS: ELISA, IHC-P, WB
APPLICATIONS: ELISA: antibody detection limit dilution 1:16000. Western Blot: Approx. 28kDa band observed in Human Skin lysates (calculated MW of 27.8kDa according to NP_006133.1). Recommended concentration: 0.03-0.1μg/ml. Immunohistochemistry: In paraffin embedded Human Skin shows strong nuclear staining in selected cells of the basal layer. Recommended concentration, 2-4μg/ml.
POSITIVE CONTROL:1) Cat. No. 1376 - Human Skin Tissue Lysate

psi-iconProperties

PURIFICATION:14-3-3 sigma antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
CLONALITY:Polyclonal
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:14-3-3 sigma antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
CONCENTRATION:500 ug/mL
STORAGE CONDITIONS:Aliquot and store at -20˚C. Minimize freezing and thawing.

psi-iconAdditional Info

OFFICIAL SYMBOL:SFN
ALTERNATE NAMES:SFN, stratifin, HGNC:10773, 14-3-3 sigma, HME1
ACCESSION NO.:NP_006133.1
PROTEIN GI NO.:5454052
GENE ID:2810

psi-iconBackground and References

REFERENCES:1) Lodygin D, Hermeking H. The role of epigenetic inactivation of 14-3-3sigma in human cancer. Cell Res. 2005 Apr;15(4):237-46. Review.

ANTIBODIES FOR RESEARCH USE ONLY.

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