Datasheet

14-3-3 sigma Antibody
CATALOG NUMBER: 33-020

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:WB
APPLICATIONS:Western blot: 1-3 ug/ml

Provided assay concentrations are suggestions only, 14-3-3 sigma antibody titration may be required for optimal results.
USER NOTE:Optimal dilutions for each application to be determined by the researcher
IMMUNOGEN:A recombinant protein fragment from the internal region of 14-3-3 sigma was used as the immunogen for this antibody. The amino acid sequence is common to the ~30kDa and ~20kDa isoforms.
HOST SPECIES:Rabbit

Properties

PURIFICATION:Protein A affinity chromatography
PHYSICAL STATE:Liquid
BUFFER:PBS with 0.1 mg/ml BSA and 0.05% sodium azide
CONCENTRATION:0.5 mg/mL
STORAGE CONDITIONS:Aliquot and Store at -20C. Avoid freez-thaw cycles.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:14-3-3 protein sigma, Epithelial cell marker protein 1, Stratifin, SFN, HME1
OFFICIAL SYMBOL:SFN
GENE ID:2810

Background

BACKGROUND:14-3-3 proteins are regulators of a diverse group of signaling proteins, including kinases, phosphatases, and transmembrane receptors, and have roles in a wide range of physiological processes including transcription, intracellular signaling, cell cycle control and apoptosis. There are seven 14-3-3 proteins in most mammals: beta, gamma epsilon, zeta, eta, sigma and tau. 14-3-3 sigma, also called Stratifin, is a product of the SFN gene. It is a tumor suppressor gene inactivated via DNA methylation. Reduced or no expression of 14-3-3 sigma is seen in various carcinoma including skin, breast and prostate, suggesting that 14-3-3 gene silencing may be involved in tumor progression.

For Research Use Only