Datasheet

ZIPK Antibody
CATALOG NUMBER: 2067

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, ICC, IF, WB
APPLICATIONS:ZIPK antibody can be used for detection of DNase II expression by Western blot at 1 μg/mL. An approximate 40 kDa band can be detected, which represents the pro-enzyme of DNase II. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1201 - HeLa Cell Lysate
 2) Cat. No. 1205 - Jurkat Cell Lysate
 3) Cat. No. 1306 - Human Spleen Tissue Lysate
 4) Cat. No. 1208 - THP-1 Cell Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 26 kDa

Observed: 27 kDa
IMMUNOGEN:ZIP kinase antibody was raised against a peptide corresponding to amino acids near the center of human ZIP kinase.

The immunogen is located within amino acids 270 - 320 of ZIPK.
HOST SPECIES:Rabbit

Properties

PURIFICATION:ZIPK Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:ZIPK Antibody is supplied in PBS containing 0.02% sodium azide.
STORAGE CONDITIONS:ZIPK antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:ZIPK Antibody: ZIP, ZIPK, Death-associated protein kinase 3, DAP-like kinase, DAP kinase 3
ACCESSION NO.:BAA81746
PROTEIN GI NO.:5162884
OFFICIAL SYMBOL:DAPK3
GENE ID:1613

Background

BACKGROUND:ZIPK Antibody: Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases. Certain serine/threonine protein kinases, such as ASK-1 and RIP, are mediators of apoptosis. A novel serine/threonine kinase that mediates apoptosis was recently identified and designated ZIP kinase. ZIP kinase contains an N-terminal kinase domain and a C-terminal leucine zipper structure and binds to ATF4 that is a member of ATF/CREB family. ZIP kinase has high sequence homology to DAP kinase (death-associated protein kinase), which is a mediator of apoptosis induced by gamma interferon. Overexpression of ZIP kinase induces apoptosis. ZIP and DAP kinases represent a novel kinase family, which mediates apoptosis through their catalytic activities. The messenger RNA was ubiquitously expressed in various tissues.
REFERENCES: 1) Kawai T, Matsumoto M, Takeda K, Sanjo H, Akira S. ZIP kinase, a novel serine/threonine kinase which mediates apoptosis. Mol Cell Biol 1998;18:1642-51 (RD1299)

For Research Use Only