Datasheet

TCTN3 Antibody
CATALOG NUMBER: 5837

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:H, M, R
TESTED APPLICATIONS:E, WB, IHC, IF
APPLICATIONS:TCTN3 antibody can be used for detection of TCTN3 by Western blot at 1-2 μg/mL. Antibody can also be used for immunohistochemistry starting at 4 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1220-SK-N-SH Cell Lysate
IMMUNOGEN:TCTN3 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human TCTN3.
HOST SPECIES:Rabbit

Properties

PURIFICATION:TCTN3 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:TCTN3 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:TCTN3 antibody can be stored at 4˚C for three months and-20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:This is a polyclonal TCTN3 Antibody.
ISOTYPE:IgG
CONJUGATE:None

Additional Info

ALTERNATE NAMES:TCTN3 Antibody: OFD4, TECT3, JBTS18, C10orf61, PSEC0041, UNQ1881/PRO4324, Tectonic-3
ACCESSION NO.:NP_056446
PROTEIN GI NO.:221136774

Background

BACKGROUND
TCTN3 Antibody: TCTN3 is a member of the Tectonic protein family, a group of evolutionarily conserved secreted and transmembrane proteins that regulate the Hedgehog (Hh)-mediated patterning of the neural tube. While the expression pattern and function of TCTN3 is not known, it is 58% similar to TCTN1. TCTN1 is expressed during embryonic development in regions that participate in Hh signaling, beginning in the gastrulation stages in the ventral node. Mice expressing mutant TCTN1 die between E13.5 and E16.5 and display holoprosencephaly, a defect associated with reduced Hh signaling, indicating the role of TCTN1 as an Hh activator. At later stages in development, TCTN1 is thought to also act as a repressor on the Hh pathway in the anterior and posterior neural tube.
REFERENCES
1) Reiter JF and Skarnes WC. Tectonic, a novel regulator of the Hedgehog pathwy required for both activation and inhibition. Genes Dev.2006; 20:22-7.

For Research Use Only