Datasheet

SIRP alpha Antibody
CATALOG NUMBER: 1125

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Bovine: (88%)
TESTED APPLICATIONS:ELISA, ICC, WB
APPLICATIONS:SIRP alpha antibody can be used for Western blot at 0.5 - 1 μg/mL. 75 - 110 kDa bands should be detected. Antibody can also be used for immunocytochemistry starting at 1 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1208 - THP-1 Cell Lysate
 2) Cat. No. 17-008 - THP-1 Cell Slide
PREDICTED MOLECULAR WEIGHT:75 - 110 kDa
SPECIFICITY:Recognizes SIRP alpha 1, 2 and 3.
IMMUNOGEN:SIRP alpha antibody was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human SIRP alpha 1.

The immunogen is located within the last 50 amino acids of SIRP alpha.
HOST SPECIES:Rabbit

Properties

PURIFICATION:SIRP alpha Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:SIRP alpha Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:SIRP alpha antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:SIRP alpha Antibody: BIT, MFR, P84, SIRP, MYD-1, SHPS1, CD172A, PTPNS1, BIT, MYD1, Tyrosine-protein phosphatase non-receptor type substrate 1, Brain Ig-like molecule with tyrosine-based activation motifs, SHP substrate 1
ACCESSION NO.:NP_542970
PROTEIN GI NO.:18426911
OFFICIAL SYMBOL:SIRPA
GENE ID:140885

Background

BACKGROUND:SIRP alpha Antibody: Protein tyrosine phosphatases (PTPases) SHP-1 and SHP-2 are critical regulators in the intracellular signaling pathways that result in cell responses such as mitosis, differentiation, migration, survival, transformation or death. SHP-2 is a signal transducer for several receptor tyrosine kinases and cytokine receptors. A novel SHP-2 associated glycoprotein was recently cloned from human, rat, mouse and cattle by several labs and was designated SIRPalpha, SHPS-1, MyD-1, BIT and p84. SIRPalpha is a new gene family containing at least fifteen members. SIRPalpha is a substrate of many activated tyrosine kinases such as insulin receptor, EGFR, PDGFR and src, and a specific docking protein for SHP-2. SIRPalpha has regulatory effects on cellular responses induced by serum, growth factors, insulin, oncogenes, growth hormones and cell adhesion and plays a general role in different physiological and pathological processes.
REFERENCES: 1) Kharitonenkov A, et al. Nature 1997;386:181-186.
2) Fujioka Y, et al. Mol Cell Biol 1996;16:6887-6899
3) Yamao T, et al. Biochem Biophys Res Commun 1997;231:61-67
4) Brooke GP, et al. Eur J Immunol 1998;28:1-11

For Research Use Only