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Primary Antibodies
p53DINP1 Antibody
Background
Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible gene was identified recently and designated p53DINP1 (for p53-dependent damage-inducible nuclear protein 1) and SIP (for stress induced protein) in human and mouse (1,2).?A p53DINP1 antisense oligonucleotide inhibits and overexpression of p53DINP1 enhances Ser46 phosphorylation of p53, induction of p53AIP1, and cell death induced by DNA double-strand breaks (1). p53DINP1 may regulate p53-dependent apoptosis through phosphorylation at Ser46 and induction of p53AIP1. The p53DINP1/SIP gene encodes two proteins of 27 and 18 kDa in human and mouse termed p53DINP1-a and p53DINP1-b or SIP27 and SIP18 (1,2). p53DINP1/SIP is expressed in many tissues and induced by a variety of stress agents including UV stress, mutagenic stress, heat shock, and oxidative stress (2).
Additional Names
p53DINP1, SIP
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Description
Left: Western blot analysis of p53DINP1 expression in human lung tissue lysate with p53DINP1 antibody at (A) 0.5 and (B) 1 μg/ml.
Below: Immunohistochemical staining of mouse liver using p53DINP1 antibody at 2 μg/ml.
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Other Product Images
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Source
p53DINP1 antibody was raised with a synthetic peptide corresponding to 14 amino acids near the amino terminus of human p53DINP1.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
p53DINP1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human p53DINP1 Peptide (Cat. No. 3045P)
Application
p53DINP1 antibody can be used for detection of p53DINP1 by Western blot at 1 to 2 μg/ml. Human lung tissue lysate can be used as a positive control and a band at 27 kDa can be detected. A lower band at 18 kDa was detected in human spleen, and mouse liver and kidney tissue lysates, which may represent the p53DINP1-b form. It is human, mouse, and rat reactive.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3045P - p53DINP1 Peptide
Storage
p53DINP1 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1302 - Human Lung Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
61216823
Protein Accession Number
Q96A56
Short Description
Apoptosis-associated p53 target
This product belongs to the following categories:
Related Products
References- Okamura S, Arakawa H, Tanaka T, Nakanishi H, Ng CC, Taya Y, Monden M, Nakamura Y. p53DINP1, a p53-inducible gene, regulates p53-dependent apoptosis. Mol Cell. 2001;8(1):85-94.
- Tomasini R, Samir AA, Vaccaro MI, Pebusque MJ, Dagorn JC, Iovanna JL, Dusetti NJ. Molecular and functional characterization of the stress-induced protein (SIP) gene and its two transcripts generated by alternative splicing. SIP induced by stress and promotes cell death. J Biol Chem. 2001;276(47):44185-92.
Datasheet 08-02W
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