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Primary Antibodies
cyclin H Antibody
Background
Cyclin H regulates CDK7, the catalytic subunit of the CDK- activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDC2/CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive carboxyl-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. It is involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.
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Description
Left: Western blot analysis of Cyclin H expression in Jurkat (A), Raji (B), and 293T (C) cell lysates using anti-Cyclin H.
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Purification
Rabbit IgG is purified by Protein A/G affinity chromatography method.
Clonality / Clone
This is a polyclonal antibody.
Host
cyclin H antibody was raised in rabbit.
Please use anti-rabbit IgG secondary antibodies.
Immunogen
cyclin H antibody was produced in rabbits immunized with a synthetic peptide corresponding to a region of human CCNH.
Application
cyclin H antibody can be used for Western blot at a suggested dilution of 1 μg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody. The protein size is 323 amino acids.
Tested Application
WB
Buffer
Antibody is supplied in PBS buffer with 2% sucrose. Product may ship lyophilized. To reconstitute add 100μl of distilled water making the final concentration 1 mg/ml.
Storage
cyclin H antibody is supplied in PBS buffer containing 0.02% sodium azide. For longer periods of storage, store at -20ºC. Avoid repeat freeze-thaw cycle.
Positive Control
- Cat. No. 1205 - Jurkat Cell Lysate
- Cat. No. 1207 - Raji Cell Lysate
- Cat. No. 1210 - 293 Cell Lysate
Species Reactivity
H
Protein Accession Number
NP_001230
Molecular Weight
37 kDa
Short Description
Anti-cyclin H
This product belongs to the following categories:
Related Products
References- Maekelae, T.P., et al., (1994) Nature 371:254-257.
Datasheet 0802-W
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