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Primary Antibodies
cIAP Antibody
Background
Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and can be prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family that binds to and inhibits cell death proteases (1 for review). The two isoforms of c-IAP (c-IAP1 and c-IAP2) are structurally related to XIAP, containing 3 baculoviral IAP repeat (BIR) motifs that are essential and sufficient for the binding and inhibition of caspases–3, –7, (2,3). The c-IAPs can associate with the death receptor TNF-R2, and mediate the ubiquitinization of TRAF2 following the binding of TNF-α by its receptor (2,4). Omi, a negative regulator of c-IAP, inhibits its activity by catalytically cleaving c-IAP (5). Another negative regulator, Smac/DIABLO, acts by enhancing the auto-ubiquitization activity of c-IAP (6).
Additional Names
cIAP (CT), cIAP
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Description
Left: Western blot analysis of c-IAP in human lung lysate with c-IAP antibody at 1 (lane A), 2 (lane B), and 4 (lane C) μg/ml, respectively.
Below: Immunohistochemistry of cIAP in human lung cells with cIAP antibody at 10 μg/ml.
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Other Product Images
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Source
c-IAP antibody was raised against a synthetic peptide corresponding to 14 amino acids at the C-terminus of human c-IAP1 c-IAP antibody detects both c-IAP1 and c-IAP2.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
cIAP antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human cIAP (C-Terminus) Peptide (Cat. No. 3325P)
Application
cIAP antibody can be used for the detection of c-IAP by Western blot at 1 to 2 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3325P - cIAP Peptide
Storage
cIAP antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1331 - Human Lung Tissue Lysate
Species Reactivity
H, M
Protein GI Number
4502141
Protein Accession Number
NP_001157
Short Description
(CT) An apoptosis inhibitor protein
This product belongs to the following categories:
Related Products
References- Schimmer AD. Inhibitor of apoptosis proteins: translating basic knowledge into clinical practice. Cancer Res. 2004; 64:7183-90.
- Rothe M, Pan M-G, Henzel WJ, et al. The TNFR2-TRAF signaling complex contains two novel proteins related to baculoviral inhibitor of apoptosis proteins. Cell 1995; 83:1243-52.
- Deveraux QL, Leo E, Stennicke HR, et al. IAPs block apoptotic events induced by caspase-8 and cytochrome c by direct inhibition of distinct caspases. EMBO J. 1998; 17:2215-23.
- Li X, Yang Y, Ashwell JD. TNF-RII and c-IAP1 mediate ubiquitinization and degradation of TRAF 2. Nature 2002; 416:345-7.
Datasheet 08-02W
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