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Primary Antibodies
ZIPK Antibody
Background
Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases. Certain serine/threonine protein kinases, such as ASK-1 and RIP, are mediators of apoptosis. A novel serine/threonine kinase that mediates apoptosis was recently identified and designated ZIP kinase . ZIP kinase contains an N-terminal kinase domain and a C-terminal leucine zipper structure and binds to ATF4 that is a member of ATF/CREB family. ZIP kinase has high sequence homology to DAP kinase (death-associated protein kinase), which is a mediator of apoptosis induced by γ interferon. Overexpression of ZIP kinase induces apoptosis. ZIP and DAP kinases represent a novel kinase family, which mediates apoptosis through their catalytic activities. The messenger RNA was ubiquitously expressed in various tissues .
Additional Names
ZIPK (IN), ZIPK
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Description
Left: Western blot analysis of ZIP kinase in HeLa (H) and Jurkat (J) whole cell lysates with ZIP kinase antibody at 1 μg/ml.
Below: Immunocytochemistry of ZIP kinase in Jurkat cells with ZIP kinase antibody at 10 μg/ml.
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Other Product Images
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Source
ZIP kinase antibody was raised against a peptide corresponding to amino acids near the center of human ZIP kinase.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
ZIPK antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human ZIPK (Intermediate Domain) Peptide (Cat. No. 2067P)
Application
ZIPK antibody can be used for detection of DNase II expression by Western blot at 1:500 to 1:1000 dilution. An approximate 40 kDa band can be detected, which represents the pro-enzyme of DNase II.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 2067P - ZIPK Peptide
Storage
ZIPK antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1306 - Human Spleen Tissue Lysate
- Cat. No. 1208 - THP-1 Whole Cell Lysate
Species Reactivity
H, M, R
Protein GI Number
5162884
Protein Accession Number
BAA81746
Short Description
(IN) Apoptosis inducing kinase
This product belongs to the following categories:
Related Products
References- Kawai T, Matsumoto M, Takeda K, Sanjo H, Akira S. ZIP kinase, a novel serine/threonine kinase which mediates apoptosis. Mol Cell Biol 1998;18:1642-51 (RD1299)
Datasheet 08-02W
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