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Primary Antibodies
ZBRK1 Antibody
Background
ZBRK1 is a member of the Kruppel-associated box-zinc finger protein (KRAB-ZFP) protein family of transcriptional repressors. ZBRK1 has been shown to not only be up-regulated in senescent fibroblasts but also interacts with BRCA1, a transcriptional regulator with tumor suppressor activity. ZBRK1 binds to a specific sequence within the GADD45 gene, supporting the assembly of a nuclear complex containing BRCA1 and repressing its transcription. Recent experiments demonstrate that ZBRK1 can homo-oligomerize in vitro and in vivo through a C-terminal transcriptional repression domain (CTRD), and this oligomerization facilitates the ZBRK1-directed transcriptional repression through ZBRK1 response elements. These results suggest that higher order ZBRK1 oligomers may assemble through both protein-DNA and CTRD-dependent protein-protein interactions.
Additional Names
ZBRK1, Zinc finger protein 350, ZNF350
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Description
Left: Western blot analysis of ZBRK1 in A-20 lysate with ZBRK1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
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Source
ZBRK1 antibody was raised against a 20 amino acid peptide near the carboxy terminus of the human ZBRK1.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
ZBRK1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
ZBRK1 antibody can be used for detection of ZBRK1 by Western blot at 1 – 2 μg/ml.
Tested Application
E, WB
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 4817P - ZBRK1 Peptide
Storage
ZBRK1 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1288 - A20 Cell Lysate
Species Reactivity
H, M, R
Protein GI Number
59710107
Protein Accession Number
NP_067645
Short Description
Zinc finger protein 350
This product belongs to the following categories:
Related Products
References- Zheng L, Pan H, Li S, et al. Sequence-specific transcription corepressor function for BRCA1 through a novel Zinc finger protein, ZBRK1. Mol. Cell 2000; 6:757-68.
- Collins T, Stone JR, and Williams AJ. All in the family: the BTB/POZ, KRAB, and SCAN domains. Mol. Cell. Biol. 2001; 21:3609-15.
- Tan W, Kim S, and Boyer TG. Tetrameric oligomerization mediates transcriptional repression by the BRCA1-dependent Kruppel-associated Box-Zinc finger protein ZBRK1. J. Biol. Chem. 2004; 279:55153-60.
Datasheet 08-01W
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