|
Primary Antibodies
ZBED3 Antibody
Background
Axin is a key modulator of the Wnt/beta-catenin pathway and acts as a scaffold protein in phosphorylating and degrading cytoplasmic beta-catenin. ZBED3, a member of the Zinc finger BED-type domain containing superfamily, has been recently identified as an Axin-interacting protein and has been suggested to modulate the Wnt/beta-catenin signaling pathway. RNA interference (RNAi) experiments targeting ZBED3 RNA attenuated Wnt-induced beta-catenin accumulation, lymphoid enhancer binding factor-1-dependent luciferase reporter activity, and Wnt gene expression. Despite its predicted molecular weight, ZBED3 often migrates at a higher position in SDS-PAGE. This antibody is predicted to have no cross-reactivity to ZBED1 or ZBED2.
Additional Names
ZBED3 (NT), Zinc finger BED-type domain containing 3
|
Description
Left: Western blot analysis of ZBED3 in rat lung tissue lysate with ZBED3 antibody at (A) 1 and (B) 2 μg/ml.
|
Source
ZBED3 antibody was raised against a 17 amino acid peptide from near the amino terminus of human ZBED3.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
ZBED3 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
ZBED3 antibody can be used for detection of ZBED3 by Western blot at 1 - 2 μg/ml.
Tested Application
E, WB
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 5123P - ZBED3 Peptide
Storage
ZBED3 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1462 - Rat Lung Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
14150185
Protein Accession Number
NP_115743
Short Description
(NT) Zinc finger BED-type domain containing 3
This product belongs to the following categories:
References- Chen T, Li M, Ding Y, et al. Identification of Zinc-finger BED domain containing 3 (Zbed3) as a novel axin-interacting protein that activates Wnt/b-catenin signaling. J. Biol. Chem. 2009; 284:6683-9.
Datasheet 09-01W
|
|
