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Primary Antibodies
XEDAR Antibody
Background
X-linked ectodysplasin-A2 receptor (XEDAR) is a recently isolated member of the tumor necrosis factor receptor family that is highly expressed during embryonic development and binds to ectodysplatin-A2 (EDA-A2) (1). Two predominantly expressed isoforms, XEDAR-s and XEDAR-L, differ by only a 21-amino region at the juxtamembrane region of the cytoplasmic domain. Neither isoform possesses a death domain and both have been shown to act mainly through TRAF3 and TRAF6 to activate the NF-κB and JNK pathways (2). Cells transfected with XEDAR and treated with EDA-A2 cause the assembly of a secondary complex containing FADD, caspase-8 and caspase-10, leading to the activation caspase-8 and caspase-3, and finally apoptosis (3). The EDA-A2-induced apoptosis is dependent on caspase-9 activation, as various pharmacological and genetic inhibitors of caspase-8 blocked apoptosis following EDA-A2 treatment (3).
Additional Names
XEDAR, X-linked ectodysplasin-A2 receptor, EDA-A2 receptor, TNFRSF27
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Description
Left: Western blot analysis of XEDAR in 293 cell lysate with XEDAR antibody at (A) 0.5, (B) 1 and (C) 2 μg/ml.
Below: Immunohistochemistry of XEDAR in human skin tissue with XEDAR antibody at 10 μg/ml.
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Other Product Images
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Source
XEDAR antibody was raised against recombinant human XEDAR.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
XEDAR antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Recombinant human XEDAR
Application
XEDAR antibody can be used for the detection of XEDAR by Western blot at 0.5 – 1 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 3855P - XEDAR Peptide
Storage
XEDAR antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1210 - 293 Cell Lysate
- Cat. No. 1376 - Human Skin Tissue Lysate
Species Reactivity
H
Protein GI Number
37222208
Protein Accession Number
AAQ89953
Short Description
Ectodysplatin-A2 receptor
This product belongs to the following categories:
Related Products
References- Yan M, Wang LC, Hymowitz SG, et al. Two-amino acid molecular switch in an epithelial morphogen that regulates binding to two distinct receptors. Science 2000; 290:523-7.
- Sinha SK, Zachariah S, Quinones HI, et al. Role of TRAF3 and -6 in the activation of the NF-κB and JNK pathways by X-linked ectodermal dysplasia receptor. J. Biol. Chem. 2002; 277:44953-61.
- Sinha SK and Chaudhary PM. Induction of apoptosis by X-linked ectodermal dysplasia receptor via a caspase 8-dependent mechanism. J. Biol. Chem. 2004; 41873-81.
Datasheet 08-02W
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